Aberrant expression of miR-141 and nuclear receptor small heterodimer partner in clinical samples of prostate cancer

Article type: Research Article

Authors: Khorasani, Maryam^a | Teimoori-Toolabi, Ladan^b | Farivar, Taghi Naserpour^c | Asgari, Mojgan^{d; e} | Abolhasani, Maryam^{d; e} | Shahrokh, Hossein^f | Afgar, Ali^b | Kalantari, Elham^d | Peymani, Amir^{c; *} | Mahdian, Reza^{b; *}

Affiliations: [a] Department of Molecular Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran | [b] Molecular Medicine Department, Pasteur Institute of Iran, Tehran, Iran | [c] Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran | [d] Oncopathology Research Center, Iran University of Medical Sciences, Tehran, Iran | [e] Department of Pathology, Hasheminejad Kidney Center, Iran University of Medical Sciences, Tehran, Iran | [f] Department of Uro-oncology, Hasheminejad Kidney Center, Iran University of Medical Sciences, Tehran, Iran

Correspondence: [*] Corresponding authors: Reza Mahdian, Molecular Medicine Department, Pasteur Institute of Iran (IPI), No. 69, 12th Farwardin Ave, Postal Code: 1316943551, Tehran, Iran. Tel./Fax: +98 21 64112439; E-mail: [email protected],MedicalMicrobiologyResearchCenter,QazvinUniversityofMedicalSciences,Qazvin,Iran.Tel:+982833324971;E-mail:[email protected].

Abstract: BACKGROUND: Prostate cancer (PCa) is the second most common cancer in men worldwide. Currently, prostate-specific antigen (PSA) test and digital rectal exam are the main screening tests used for PCa diagnosis. However, due to the low specificity of these tests, new alternative biomarkers such as deregulated RNAs and microRNAs have been implemented. OBJECTIVES:Aberrant expressions of small heterodimer partner gene (SHP, NR0B2) and mir-141 are reported in various cancers. The aim of this study was to investigate the SHP and miR-141 expression level in tissue samples of prostate cancer. METHODS:The expression level of SHP gene and miR-141 was assessed by real time PCR and their relative amounts were calculated by the Δ⁢ΔCT method. Also, IHC technique was used to determine the expression level of SHP protein. RESULTS:The miR-141 was significantly up-regulated in the samples of metastatic tumors compared to localized tumor samples (P< 0.001, 31.17-fold change). Tumor samples showed lower SHP mRNA expression levels than BPH samples (p= 0.014, 4.7-fold change). The results of paired t-test analysis showed there was no significant difference between the SHP gene expression in PCa samples and their matched tumor-adjacent normal tissue (p= 0.5). CONCLUSIONS:The data obtained in our study confirm the involvement of miR-141 in PCa progression and metastasis. These effects could be mediated by AR via down-regulation of its co-repressor protein, i.e., SHP.

Keywords: Prostate cancer, SHP gene, miR-141, biomarker, nuclear receptors, gene expression, Immunohistochemistry

DOI: 10.3233/CBM-170696

Journal: Cancer Biomarkers, vol. 22, no. 1, pp. 19-28, 2018