Affiliations: [a] Department of Gynaecology and Obstetrics, The Second Hospital of Jilin University, Changchun 130041, Jilin, China | [b] Bethune School of Medicine, Jilin University, Changchun 130021, Jilin, China | [c] Deparment of Pathology and Pathophysiology, Bethune Medical College, Jilin University, Changchun 130021,Jilin, China | [d] Department of Pediatric Surgery, The First Hospital of Jilin University, Changchun 130021, Jilin, China
Correspondence:
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Corresponding author: Fei Wu, Department of Gynaecology and Obstetrics, The Second Hospital of Jilin University, No. 218, Ziqiang Street, Nanguan District, Changchun 130041, Jilin, China. Tel./Fax: +86 0431 88796635; E-mail: [email protected].
Abstract: OBJECTIVE: Cervical squamous cell carcinoma seriously threats to patient’s life and health. MiRNAs have role of regulating cell growth, proliferation, and death. MiRNAs can promote or inhibit cell growth and proliferation. This study discussed the role of miRNA373 in regulating cervical squamous cell carcinoma growth, proliferation, and apoptosis. PATIENTS AND METHODS: MiRNA373 and scramble miRNA were synthetized and transfected to cervical squamous cell carcinoma SiHa cells by lipofectamine. IAPs plasmid and miRNA373 were sequentially transfected to SiHa cells. MTT assay, caspase-3 activity, and flow cytometry were applied to test miRNA373 and IAPs impacts on cell growth, proliferation, and apoptosis. Western blot was adopted to determine IAPs expression. RESULTS: MiRNA373 transfection obviously reduced SiHa cell growth, induced phosphatidylserine eversion and caspase-3 activation, and declined IAPs expression. IAPs interference significantly enhanced miRNA373 induced SiHa cell apoptosis. IAPs overexpression markedly restrained miRNA373 induced SiHa cell apoptosis. CONCLUSIONS: MiRNA373 transfection suppressed SiHa cell growth and proliferation. MiRNA373 induced SiHa cell apoptosis possibly through downregulating IAPs, suggesting that IAPs might be a target for cervical squamous cell carcinoma treatment.
