Affiliations: Department of Respiration, The 411th Hospital of Chinese PLA, Second Military Medical University, Shanghai 200081, China
Correspondence:
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Corresponding author: An-Shan Zhuo, Department of Respiration, The 411th Hospital of Chinese PLA, Second Military Medical University, Shanghai 200081, China. Tel.: +86 21 81867221; Fax: +86 21 81867221; E-mail: [email protected].
Abstract: BACKGROUND: The aim in this study was to explore the role of long non-coding RNA GHET1 in development of non small cell lung cancer (NSCLC). METHODS: LncRNA GHET1 expression levels were analyzed by qRT-PCR in tumor tissues and adjacent normal tissues in NSCLC. Measuring the cell proliferation and invasion abilities by CCK8, cell colony formation and transwell invasion assays. Relative protein expression was analyzed by western blot assays. RESULTS: Expression of lncRNA GHET1 was notably higher in NSCLC tissues compared with adjacent normal tissues by using qRT-PCR analyses. Higher lncRNA GHET1 expression associated with lymph node metastasis, TNM stage and showed poor outcome in NSCLC patients. Knockdown of lncRNA GHET1 suppressed cell proliferation and invasion capacity and Epithelial-Mesenchymal Transition (EMT) phenomenon of NSCLC cells. Moreover, we demonstrated that knockdown of lncRNA GHET1 suppresses LATS1/YAP pathway signaling pathway by downregulating YAP1 expression in NSCLC cells. CONCLUSIONS: GHET1 predicted a poor outcome and acted as a tumor-promoting gene in NSCLC. Thus, inhibition of GHET1 may be a potential target of NSCLC treatment.
Keywords: Non small cell lung cancer, long non-coding RNA, GHET1, cell proliferation, cell invasion
