Wide-transcriptome analysis and cellularity of bone marrow CD34+/lin- cells of patients with chronic-phase chronic myeloid leukemia at diagnosis vs. 12 months of first-line nilotinib treatment

Article type: Research Article

Authors: Trojani, Alessandra^{a; *} | Pungolino, Ester^a | Rossi, Giuseppe^b | D’Adda, Mariella^b | Lodola, Milena^a | Camillo, Barbara Di^c | Perego, Alessandra^d | Turrini, Mauro^e | Orlandi, Ester^f | Borin, Lorenza^g | Iurlo, Alessandra^h | Malato, Simonaⁱ | Spina, Francesco^j | Latargia, Maria Luisa^k | Lanza, Francesco^l | Artale, Salvatore^m | Anghilieri, Michelaⁿ | Carraro, Maria Cristina^o | Canal, Gabriella De^p | Morra, Enrica^q | Cairoli, Roberto^a

Affiliations: [a] Division of Hematology, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy | [b] Department of Hematology, ASST Spedali Civili, Brescia, Italy | [c] Department of Information Engineering, University of Padova, Padova, Italy | [d] Internal Medicine-Haematology, Desio Hospital, Desio, Italy | [e] Division of Hematology, Department of Internal Medicine, Valduce Hospital, Como, Italy | [f] Hematology Unit, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy | [g] Hematology Division, San Gerardo Hospital, Monza, Italy | [h] Oncohematology Division, IRCCS Ca’ Granda – Maggiore Policlinico Hospital Foundation, Milano, Italy | [i] Hematology and Bone Marrow Transplantation Unit, San Raffaele Scientific Institute, Milano, Italy | [j] Division of Hematology – Fondazione IRCCS Istituto Nazionale dei Tumori, Milano, Italy | [k] ASST Valle Olona Ospedale di Circolo, Busto Arsizio, Italy | [l] Division of Hematology, Hospital of Cremona, Cremona, Italy | [m] ASST Valle Olona Sant’Antonio Abate, Gallarate, Italy | [n] ASST Lecco, Lecco, Italy | [o] Hematology and Transfusion Medicine, Sacco Hospital, Milano, Italy | [p] Pathology Department, Cytogenetics, ASST Grande Ospedale Metropolitano Niguarda, Milano, Italy | [q] Executive Committee, Rete Ematologia Lombarda, Italy

Correspondence: [*] Corresponding author: Alessandra Trojani, Division of Hematology, ASST Grande Ospedale Metropolitano Niguarda, Piazza Ospedale Maggiore, 3, 20162 Milan, Italy. Tel.: +39 2 64442711; Fax: +39 2 64447598; E-mail: [email protected].

Abstract: BACKGROUND: Chronic myeloid leukemia (CML) is a clonal myeloproliferative disorder with heterogeneous biological and clinical features. The biomolecular mechanisms of CML response to tyrosine-kinase inhibitors are not fully defined. OBJECTIVE: We undertook a gene expression profiling (GEP) study of selected bone marrow (BM) CD34+/lin- cells of chronic-phase CML patients at diagnosis and after 12 months of TKI nilotinib to investigate molecular signatures characterizing both conditions.

DOI: 10.3233/CBM-170209

Journal: Cancer Biomarkers, vol. 21, no. 1, pp. 41-53, 2018