Affiliations: [a] Department of Clinical Laboratory, The Second Hospital of Tianjin Medical University, Tianjin, China | [b] Department of Nephrology, The Second Hospital of Tianjin Medical University, Tianjin, China | [c] Department of Clinical Laboratory, The First Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi, China
Correspondence:
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Corresponding author: Li Ning, Department of Clinical Laboratory, The Second Hospital of Tianjin Medical University, No. 23 Pingjiang Street, Hexi District, Tianjin 300211, China. Tel.: +86 13516141753; E-mail:[email protected]
Abstract: BACKGROUND: The long non-coding RNAs (lncRNAs) are emerging as
important regulators in cancer progression. Clear cell renal cell carcinoma
(ccRCC) is one of the most common urological cancers with poor prognosis. In
this study, we examined the functional role of the lncRNA, nuclear enriched
abundant transcript 1 (NEAT1) in ccRCC progression. METHODS: We performed quantitative real time PCR and western
blotting assays to measure mRNA and protein expression levels, respectively.
CCK-8 assay, cell invasion and migration assays were used to determine the
cell proliferative, cell invasive and migratory ability. Flow cytometric
analysis was performed to examine cell apoptosis. RESULTS: The expression levels of NEAT1 was up-regulated in ccRCC
tissues and up-regulation of NETA1 was positively correlated with tumor
size, higher Fuhrman grade, and lymph node metastasis, and also predicts
worse 5-year survival rate of patients with ccRCC. NEAT1 knock-down by NEAT
siRNAs transfection suppressed cell proliferation and induced cell apoptosis
in ccRCC cell lines. In addition, NEAT1 knock-down suppressed cell invasion
and migration and inhibited the mRNA and protein expression levels of
epithelial-mesenchymal transition-related markers in ccRCC cell lines. CONCLUSIONS: In conclusion, NEAT1 may be an important mediator in
the regulation of ccRCC progression and predicts the poor prognosis in
patients with ccRCC.
