Affiliations: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China
Correspondence:
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Corresponding author: Ping Liu, Department of Oncology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, Jiangsu, China. Tel.: +86 25 83718836 6080; Fax: +86 25 83780826; E-mail:[email protected]
Abstract: BACKGROUND: Studies have reported the prognostic value of
dysregulated microRNAs (miRNAs) in gastric cancer (GC). However, the results
demonstrated so far are inconsistent. OBJECTIVE: To better understand the miRNAs with prognostic
relevance. METHODS: Evaluable miRNAs were selected based on our selection
criteria and further analyzed in formalin-fixed paraffin-embedded (FFPE)
tissue samples of 169 GC patients using quantitative real-time polymerase
chain reaction (qRT-PCR). RESULTS: A total of 19 miRNAs were selected as candidate miRNAs.
Among those miRNAs identified, high expression of miR-21-5p was related to
poor overall survival (OS) and disease free survival (DFS) and was
identified as an independent prognostic factor. Cases with high level of
miR-200c-3p showed poor DFS. Subgroup analysis revealed that high expression
of miR-21-5p and miR-222-3p was associated with poor OS and DFS in GC
patients not received adjuvant chemotherapy. In male patients, high
expression level of miR-21-5p was related to poor OS and DFS. CONCLUSIONS: The present study confirmed that elevated level of
miR-21-5p could serve as an independent predictor for poor OS and DFS of GC
patients. Moreover, miR-200c-3p, miR-222-3p might also play
important roles in the prognosis of GC patients. Further studies are
warranted to validate our findings and identify the functions and mechanisms
of these miRNAs.
