Affiliations: [a] Department of Anaesthesiology, King George's Medical University, Lucknow, India | [b] Department of Biochemistry, King George's Medical University, Lucknow, India | [c] Department of Surgical Oncology, King George's Medical University, Lucknow, India | [d] Department of Microbiology, King George's Medical University, Lucknow, India
Correspondence:
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Corresponding author: Girish Chandra, Department of Anaesthesiology, King George's Medical University, Lucknow 226003, India. Mob. +91 9415023391; E-mail: [email protected].
Abstract: Background:The pro-inflammatory cytokines play an essential role in immune response and are involved in a variety of inflammatory and infectious disease. Tumor necrosis factor alpha (TNF-α) gene polymorphism has been a potential determinant of susceptibility to various types of cancer. Objective:To evaluate the association of TNF-α gene promoter (-238) G/A and (-308) G/A polymorphisms with the susceptibility of OSCC patients in North Indian population. Methods:A total 272 patients with OSCC and 185 healthy volunteers were genotypes for the TNF-α (-238) G/A and (-308) G/A gene polymorphism. Genotypes were identified by polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP). Genotype frequencies were evaluated by Chi-square test and Odds ratio (OR) relative risk. Results:TNF-α (-238) G/A polymorphism was significantly associated with OSCC patients as compared to healthy volunteers (GG vs. GA: OR=0.3500, 95% CI=0.1289–09502; p=0.036; G vs. A: OR=0.3589 1.477, 95% CI=0.1335–0.9652; p=0.0386). No significant association was found in TNF-α (-308) G/A gene polymorphism with OSCC patients and controls. Conclusions:We conclude that the TNF-α (-238) G/A polymorphism was significantly associated with OSCC however TNF-α (-308) G/A polymorphism was not associated in OSCC patients.
