Affiliations: [a] Genetics of Non-Communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran | [b] Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran | [c] Department of Internal Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran | [d] Department of Genetics, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
Correspondence:
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Corresponding author: Mohammad Hashemi, Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. Tel.: +98 541 3235122; E-mail: [email protected].
Abstract: Background:Recent evidence has demonstrated the implication of CC chemokine ligand 5 (CCL5) and CC chemokine receptor 5 (CCR5) in breast tumor initiation and progression. Objective:The purpose of this study was to investigate whether single nucleotide polymorphisms of CCL5 -403 G>A (rs2107538) and CCR5 Δ32 genes are associated with the breast cancer (BC) risk. Methods:A total of 439 subjects including on 236 BC patients and 203 healthy controls from the same area were recruited. The CCL5 -403 G>A and CCR5 Δ32 polymorphisms were genotyped by allele-specific polymerase chain reaction (AS-PCR) and PCR, respectively. Results:Our data demonstrated that the CCL5 -403 GA and GA+AA genotypes, with a higher frequency in the BC patients compared to the control group, were associated with an increased risk of BC in the codominant (GG vs. GA OR=1.75, 95%CI=1.07–2.86, P=0.025) and dominant models (GG vs. GA+AA: OR=1.84, 95%CI=1.15–2.93, P=0.014), respectively. Additionally, the A allele of CCL5 -403 G>A variation was found more prevalent in the BC patients than in controls (14% vs. 8%) and was a risk factor for BC (G vs. A: OR=1.87, 95% CI=1.21–2.89, P=0.004). Conclusions:Our findings highlighted that the CCL5 -403 G>A polymorphism is a risk factor for BC in our population. Our findings suggest that the CCL5 -403 GA and GA+AA genotypes and the A allele were associated with an elevated risk of BC which may function as risk factor for breast carcinoma.
Keywords: CCL5, CCR5, breast cancer, gene polymorphism
