Tumorigenesis and tumor progression related gene expression profiles in colorectal cancer

Article type: Research Article

Authors: Huang, Ming-Yii^{a; b; 1} | Chen, Hung-Chang^{c; 1} | Yang, I-Ping^{d; e} | Tsai, Hsiang-Lin^{f; g} | Wang, Tsu-Nai^h | Juo, Suh-Hang Hank^{d; i} | Wang, Jaw-Yuan^{d; f; g; *}

Affiliations: [a] Department of Radiation Oncology and Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan | [b] Department of Radiation Oncology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan | [c] Division of Colorectal Surgery, Department of Surgery, Changhua Christian Hospital, Changhua, Taiwan | [d] Department of Medical Genetics, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan | [e] Department of Nursing, Shu-Zen College of Medicine and Management, Kaohsiung, Taiwan | [f] Department of Surgery, Faculty of Medicine, Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan | [g] Division of Gastroenterology and General Surgery and Cancer Center, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan | [h] Department of Public Health, College of Health Science, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan | [i] Department of Medical Research and Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

Correspondence: [*] Corresponding author: Jaw-Yuan Wang, Department of Surgery, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung 80756, Taiwan. Tel.: +886 7 312 1101 (ext.7158); Fax: +886 7 322 2110; E-mail: [email protected].

Note: [1] Ming-Yii Huang and Hung-Chang Chen contributed equally to this study.

Abstract: Background:To investigate the different gene expression profiles in colorectal cancer (CRC) has important implications in understanding the correlation between candidate genes and clinical histopathological features, as well as in developing prognostic prediction markers. Objective:The purpose of this study was to identify the expression profiles of tumorigenesis and tumor progression related genes in Taiwanese CRC patients. Methods:In this study, we analyze 18 candidate gene expressions of 77 CRC tissues by a GeXP multiplexed assay. Results:The results showed VEGF (71.4%), SOX9 (68.8%), MYC (62.3%), CCND1 (59.7%), TP53 (59.4%), MMP9 (53.3%), and BIRC5 (50.6%) as significantly overexpressed genes in CRC tissues. VEGF was the most highly overexpressed gene. In addition, VEGF was overexpressed in larger tumors (P=0.037, OR=2.981, 95% CI, 1.049–8.477). MMP9 overexpression was correlated to deeper tumor invasion (P=0.001, OR=11.022, 95% CI, 2.281–53.262), and BIRC5 was overexpressed in the presence of perineural invasion (P=0.026, OR=4.250, 95% CI, 1.240–14.562). Conclusions:The results of this study offered valuable information about the relationship between different gene expressions and clinical pathological features, and these biomarkers represent a potential role for CRC prognosis prediction and establishing therapeutic strategies.

Keywords: Tumorigenesis, tumor progression, colorectal cancer, GeXP multiplexed assay

DOI: 10.3233/CBM-130350

Journal: Cancer Biomarkers, vol. 13, no. 4, pp. 269-279, 2013