Overexpression of Aldo-keto reductase family 1 B10 protein in ductal carcinoma in situ of the breast correlates with HER2 positivity

Issue title: Adrenergic Signaling and Cancer: Deciphering the Connections

Guest editors: Amal Melhem-Bertrandtx and Anil K. Soody

Article type: Research Article

Authors: Li, Zezhong^{a; c; 1} | He, Xin^{b; 1} | Xing, Shenyang^{a; c} | Ni, Jinsong^b | Zhang, Weihong^b | Xu, Xiaofang^a | Gao, Feng^{a; c} | Gai, Jinhong^a | Zhao, Zhihui^{a; c} | Li, Jianhua^a | Gong, Pengtao^a | Zhang, Guocai^a | Zhang, Xichen^{a; *}

Affiliations: [a] College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin, China | [b] Department of pathology, First Hospital of Jilin University, Jilin University, Changchun, Jilin, China | [c] The Center of Animal Embryo Engineering of Jilin Province, Jilin University, Changchun, Jilin, China | [x] Department of Breast medical Oncology, M.D. Anderson Cancer Center, Houston, TX, USA | [y] Department of Gynecologic Oncology, M.D. Anderson Cancer Center, Houston, TX, USA

Correspondence: [*] Corresponding author: Xichen Zhang, College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun 130062, Jilin, China. Tel.: +86 431 87981351; Fax: +86 431 87981351; E-mail: [email protected].

Note: [1] These authors contributed equally to this work.

Abstract: Background:Ductal carcinoma in situ of the breast constitutes the early stage of breast cancer when cancer cells are confined by the intact myoepithelial cell layer. Transition from DCIS to invasive carcinoma is a process yet poorly understood. Materials and Methods:By liquid chromatography (LC) and mass spectrometry (MS/MS) methods, we analyzed this early event using paired samples of micro-dissected cells overlaid with focally disrupted myoepithelial layers and their adjacent counterparts within the intact duct from formalin-fixed paraffin-embedded blocks. Results:AKR1B10, a member of Aldo-keto reductase family, was shown to be abundantly located in the filtering cells among a catalog of proteins. Moreover, strong correlation between AKR1B10 and HER2 positivity was found in an independent cohort of DCIS samples. Conclusion:AKR1B10 could become a potential diagnosis and therapeutic marker for early breast cancers with HER2 overexpression and poor prognosis.

Keywords: AKR1B10, HER2, correlation, DCIS

DOI: 10.3233/CBM-130337

Journal: Cancer Biomarkers, vol. 13, no. 3, pp. 181-192, 2013