Association of LEP and ADIPOQ common variants with colorectal cancer in Mexican patients

Article type: Research Article

Authors: Partida-Pérez, Miriam^a | de la Luz Ayala-Madrigal, María^a | Peregrina-Sandoval, Jorge^b | Macías-Gómez, Nelly^c | Moreno-Ortiz, José^a | Leal-Ugarte, Evelia^d | Cárdenas-Meza, Mario^e | Centeno-Flores, Manuel^e | Maciel-Gutiérrez, Víctor^e | Cabrales, Enrique^f | Cervantes-Ortiz, Sergio^{f; g} | Gutiérrez-Angulo, Melva^{g; *}

Affiliations: [a] Instituto de Genética Humana, CUCS, U de G. Sierra Mojada no. 950, Guadalajara, Jalisco, México | [b] Laboratorio de Inmunobiología, CUCBA, U de G. Carretera Gdl-Nogales km 15.5 predio las Agujas, Zapopán, Jalisco, México | [c] Laboratorio de Genética Humana, CUSur, U de G. Prolongación Colón S/N, Ciudad Guzmán, Jalisco, México | [d] Unidad Académica de Ciencias de la Salud y Tecnología, UAT, Sendero Nacional km 3, Matamoros, Tamaulipas, México | [e] Hospital Civil de Guadalajara “Dr. Juan I. Menchaca”, Salvador de Quevedo y Zubieta N° 750. Guadalajara, Jalisco, México | [f] Hospital Civil de Guadalajara “Fray Antonio Alcalde”, Hospital #278, Guadalajara, Jalisco, México | [g] Departamento de Clínicas, CUAltos, U de G. Carretera a Yahualica km 7.5, Tepatitlán de Morelos, Jalisco, México

Correspondence: [*] Corresponding author: Melva Gutiérrez-Angulo, PhD, Departamento de Clínicas, CUAltos, U de G. Carretera a Yahualica km 7.5, Tepatitlán de Morelos, Jalisco, México, Tel.: +52 3787828033 ext. 6975; E-mail: [email protected].

Abstract: Leptin and adiponectin are cytokines produced by adipose tissue with opposite effects on tumor growth: the former stimulate whereas the latter inhibit it. The objective was to analyze the association of LEP A19G and ADIPOQ+45 T/G and +276 G/T polymorphisms in Mexican patients with colorectal cancer (CRC). 68 unrelated patients with CRC (study group) and 102 blood donors (control group); all subjects were Mestizos from western Mexico. The polymorphisms were established by PCR-RFLP on DNA samples obtained from peripheral blood. The LEP A19G polymorphism showed significant differences between CRC patients and control group (p= 0.01 for G/A genotype and p= 0.02 for the recessive model G/G +G/A); yet, in the analysis stratified by gender, this difference remained significant only in males. The ADIPOQ polymorphisms did not shown any significant differences. Our results suggest that the A19G LEP polymorphism is associated with CRC in Mexican patients.

Keywords: Leptin, adiponectin, colorectal cancer, polymorphism

DOI: 10.3233/CBM-2010-0154

Journal: Cancer Biomarkers, vol. 7, no. 3, pp. 117-121, 2010