Affiliations: Cell Biology and Biochemistry, Pacific Northwest National Laboratory, Richland, WA, USA | Program in Molecular Biology and Genetics, Karmanos Cancer Institute and Department of Pathology, Wayne State University School of Medicine, 110 E. Warren, Detroit, MI, USA
Correspondence:
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Corresponding author: Richard Zangar, Pacific Northwest National Laboratory, 902 Battelle Boulevard, Richland, WA 99352 USA. Tel.: +1 509 371 7301; Fax: +1 509 371 7304; E-mail: [email protected].
Abstract: Enzyme-linked immunosorbent assay (ELISA) microarray technology promises to be a powerful tool for detecting and validating protein biomarkers, especially panels of biomarkers. ELISA microarrays are capable of high-throughput analysis of multiple proteins using small sample volumes. In this chapter we review the literature on the use of antibody microarrays for biomarker discovery and validation. We also described the methodologies we employ to obtain high-quality data through protocol optimization and data calibration.
