Affiliations: [a] Department of Biochemistry, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia | [b] Graduate School of Hasanuddin University, Makassar, Indonesia | [c] Department of Anatomic Pathology, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia | [d] Department of Public Health, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Correspondence:
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Corresponding author: Ika Yustisia, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Tamalanrea Campus, 90245, Makassar, South Sulawesi, Indonesia. Tel.: 0411 586010; E-mail: [email protected], [email protected]
Note: [†] These authors share first co-authorship to this manuscript.
Abstract: BACKGROUND:The role of gluconeogenesis in cancer cells as the reverse pathway for glycolysis is not well known. Several studies of gluconeogenesis in cancer cells still show conflicting results. Expression of key enzymes such as FBP1 and LDHB in cancer tissues may explain the role of gluconeogenesis in tumor development. OBJECTIVE:This study aimed to analyze the expression of FBP1 and LDHB in fibroadenomas and invasive cancers of the breast. METHODS:The immunohistochemical staining technique was used to show the expression of FBP1 and LDHB in formalin-fixed, paraffin-embedded blocks of 32 fibroadenomas and 31 invasive breast cancer samples. RESULTS:FBP1 was expressed by the majority of fibroadenoma (68.7%) and invasive breast cancer (71%) samples. LDHB expression in fibroadenomas was significantly higher than in invasive breast cancers (P = 0.029). The expression of these two enzymes was found in invasive, lobular, and tubular breast carcinoma, and at well, moderately, and poorly differentiated breast malignancy. CONCLUSIONS:High expression of FBP1 and LDHB was found in fibroadenomas and invasive breast cancers. A higher level of LDHB expression was observed in fibroadenomas. These results may indicate the enzymes’ role in the pathogenesis of both breast diseases.
Keywords: FBP1, LDHB, gluconeogenesis, fibroadenomas, breast cancers
DOI: 10.3233/BD-201035
Journal: Breast Disease, vol. 40, no. 4, pp. 251-256, 2021
