Affiliations: [a] Department of Virology, Iran University of Medical Sciences, Tehran, Iran | [b] Department of Breast Surgery, Iran University of Medical Science, Rasool-Akram Hospital, Tehran, Iran | [c] Department of Pathology, Shahid Beheshti University of Medical Sciences, Imam Hossein Hospital, Tehran, Iran | [d] Department of Anesthesia, Iran University of Medical Sciences, Tehran, Iran
Correspondence:
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Corresponding author: Seyed Hamidreza Monavari, Department of Virology, Iran University of Medical Sciences, Tehran, Iran. E-mail: [email protected]
Abstract: BACKGROUND:The etiology and molecular mechanisms involved in the development of breast cancer still remain poorly understood. Some epidemiological studies have shown an association between human papillomavirus (HPV) and breast cancer. However, the findings are controversial. OBJECTIVE:Our study was aimed to investigate the presence of HPV DNA in breast carcinomas of Iranian women. METHODS:In total, 72 samples of formalin-fixed paraffin-embedded (FFPE) tissues of breast cancer collected between December 2014 and April 2016 were examined. HPV DNA detection was performed by nested-PCR assay. Next, positive samples were subjected to genotyping by the CLART HPV2 microarray system. All statistical analysis was carried out using SPSS v.18.0. RESULTS:HPV DNA was detected in 4/72 (5.55%) samples. Clinical factors were not statistically associated with HPV presence. However, CLART HPV2 microarray assay failed to determine the genotype of any positive samples. CONCLUSION:The low frequency of HPV detected in our study does not support an association between breast carcinoma and HPV infection. However, it is possible that HPV may be responsible for breast carcinogenesis only in small percentage of all breast cancer.
Keywords: Human papillomavirus, Breast carcinoma, Iran, HPV, Breast cancer
DOI: 10.3233/BD-180333
Journal: Breast Disease, vol. 37, no. 4, pp. 207-213, 2018
