The High Lead Level Role in the DNA Repair RAD 18 and OGG1 Gene Polymorphism in Gasoline Station Workers

Article type: Research Article

Authors: Hasan, Aizhar Hamzih | Asker Aljuboory, Dhuha Salman | Hussein, Hawraa Hamid | Al-Terehi, Mona N.^{; *}

Affiliations: Department of Biology/Biotechnology, College of Science, University of Babylon, Hillah, Iraq | Department of Medical Laboratory Techniques, Al-Mustaqbal University College, Hillah, Iraq | Department of Physiology and Medical Physics, College of Medicine, University of Al-Ameed, Karbala, Iraq

Correspondence: [*] Corresponding Author. [email protected]

Abstract: The DNA repair enzymes–heavy metal interaction is an interesting project that can help elucidate several diseases. The current study aims to assess the lead effect in the two DNA repair genes RAD-18 Arg302Gln (rs373572) and OGG1 Ser326Cys in Gasoline station workers. The output showed that work types were Gasoline supply worker with a high percentage (73.52%) than other groups (11.76% and 14.7%) for a maintenance worker and station employee, respectively, significant lead (p 0.010) increasing in station worker than the control group, and significant lead changes among work types groups (p 0.000), the station employee has a low level of lead than other groups, while the Gasoline supply worker has a higher level than other groups, the RAD 18 gene showed two polymorphisms Gln/Gln and Gln/Arg, and OGG1 showed two haplotypes, single and double haplotypes, non-significant association although of high frequent of Glu/Arg in the gasoline supply worker, and significant association of allele frequency, significant association with station worker that have two types of haplotypes (single and double haplotypes) while lack of tri-haplotypes which prepared in higher percentage in control group. The lead level according to RAD 18 genotyping show non-significant (p 0.454) elevation in Gln/Arg genotyping, and according to OGG 1 haplotype lead level was non-significant, changed (p 0.481) between single and double haplotypes. From these outputs, it can be concluded that the lead level is a significant elevation in gasoline station worker and it did not affect RA18 and OGG 1 genotyping, the RAD18 did not associate with workers while OGG strongly associated with them.

Keywords: Lead level, DNA repair, RAD 18, OGG1, gene polymorphism, gasoline station workers

DOI: 10.3233/AJW230020

Journal: Asian Journal of Water, Environment and Pollution, vol. 20, no. 2, pp. 17-22, 2023