Affiliations: [a] Departamento de Morfología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes. Av. Universidad #940. Ciudad universitaria. Aguascalientes, Ags. México | [b] Departamento de Química, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes. Av. Universidad #940. Ciudad universitaria. Aguascalientes, Ags. México | [c] Departamento de Biología de la Reproducción, Universidad Autónoma Metropolitana, Unidad Iztapalapa. Av. San Rafael Atlixco No. 186. Col. Vicentina, Del. Iztapalapa. D.F. México | [d] Departamento de Fisiología y Farmacología, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes. Av. Universidad #940. Ciudad universitaria. Aguascalientes, Ags. México | [e] Sección de Estudios de Posgrado e Investigación, ESM-IPN, México
Correspondence:
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Correspondence to: Javier Ventura-Juárez, PhD, Departamento de Morfología, Centro de Ciencias Básicas, Avenida Universidad 940, Colonia Ciudad Universitaria, edificio 202, Aguascalientes, Postal Code 20131, México. Tel.: +52 449 9108425; E-mail: [email protected].
Abstract: An inflammation is generated during formation of Amoebic Liver Abscess. We analyzed chemically sympathectomized hamsters with Amoebic Liver Abscess, in a period between 6 hours to 7 days. The liver tissue samples were analyzed by haematoxylin and eosin staining, immunohistochemistry, immunofluorescence and morphometry. Sympathectomy caused a lower production of collagen and absence of granuloma tissue of Amoebic Liver Abscess. At the 6 h of development, proinflammatory cytokines were reduced, also reduced 12 hours – 7 days; while IL-10 production was incresed for this time; Transforming Growth Factor-β producing cells only increased from 4 to 7 days. Nuclear factor kappa-light-chain-enhancer of activated B cells were diminished throughout the Amoebic Liver Abscess, while Toll Like Receptor 4 + macrophages decreased in the period of 2 to 7 days; and Toll Like Receptor 4 + neutrophils decreased between 4 and 7 days. The population of trophozoites was increased in sympathectomized animals between 4 and 7 days. The chemical sympathectomy reduces the collagen deposition and induces an anti-inflammatory state during the development of Amoebic Liver Abscess, then; allows the spread and reproduction of Entamoeba histolytica trophozoites.
