Effects of Bushen Huoxue on integrin β3 and integrin β5 in the placental tissue of mice with repeated implantation failure caused by phospholipid antibodies

Article type: Research Article

Authors: Han, Yongmei^{a; b; 1; *} | Liu, Xinyu^{a; b; 1} | Meng, Xinwan^{a; b; *}

Affiliations: [a] Department of Reproductive Medicine, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, China | [b] The First Clinical Medical College of Henan University of Chinese Medicine, Zhengzhou, China

Correspondence: [*] Corresponding authors: Yongmei Han and Xinwan Meng, Department of Reproductive Medicine, The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, China. E-mail: [email protected] and [email protected].

Note: [1] Contributed equally.

Abstract: BACKGROUND: This study aimed to investigate the effects of the Kidney-Tonifying and Blood-Activating Formula on combating the downregulation of integrin β3 and integrin β5 in mouse placental tissue induced by phospholipid antibodies. OBJECTIVE: This study aimed to investigate the effects of kidney-tonifying and blood-activating formulations on combating the downregulation of integrin β3 and integrin β5 in mouse placental tissue induced by phospholipid antibodies. METHODS: Mice in the phospholipid antibody group and phospholipid antibody + kidney-tonifying and blood-activating formula group underwent repeated implantation experiments, and the expression of integrin β3 and integrin β5 in placental tissue was observed. The effects of the kidney-tonifying and blood-activating formula on mouse placental tissue were evaluated through biochemical index tests, histopathological observations, and immunohistochemical staining. RESULTS: After intervention with the kidney-tonifying and blood-activating formula, the expression of integrin β3 and integrin β5 in placental tissue was notably upregulated, and placental structure was restored. Kidney-Tonifying and Blood-Activating Formula significantly improved abnormal biochemical indices and exerted a remarkable protective effect on placental tissue pathology. CONCLUSION: The kidney-tonifying and blood-activating formula effectively counteracted the downregulation of integrin β3 and integrin β5 in mouse placental tissue induced by phospholipid antibodies and alleviated placental tissue pathology through structural improvement and protection. Therefore, kidney-tonifying and blood-activating formulas may serve as potential therapeutic agents for recurrent implantation failure, offering new insights and approaches for clinical treatment.

Keywords: Kidney-tonifying and blood-activating formula, phospholipid antibodies, recurrent implantation failure, placental tissue, integrin β3, integrin β5

DOI: 10.3233/THC-241179

Journal: Technology and Health Care, vol. Pre-press, no. Pre-press, pp. 1-13, 2024