Journal of Berry Research - Volume Pre-press, issue Pre-press
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Impact Factor 2020: 2.208
The main objective of the
Journal of Berry Research is to improve the knowledge about quality and production of berries to benefit health of the consumers and maintain profitable production using sustainable systems.
The objective will be achieved by focusing on four main areas of research and development:
1. From genetics to variety evaluation
2. Nursery production systems and plant quality control
3. Plant physiology, biochemistry and molecular biology, as well as cultural management
4. Health for the consumer: components and factors affecting berries' nutritional value
Specifically, the journal will cover berries (strawberry, raspberry, blackberry, blueberry, cranberry currants, etc.), as well as grapes and small soft fruit in general (e.g., kiwi fruit). It will publish research results covering all areas of plant breeding, including plant genetics, genomics, functional genomics, proteomics and metabolomics, plant physiology, plant pathology and plant development, as well as results dealing with the chemistry and biochemistry of bioactive compounds contained in such fruits and their possible role in human health. Contributions detailing possible pharmacological, medical or therapeutic use or dietary significance will be welcomed in addition to studies regarding biosafety issues of genetically modified plants.
Journal of Berry Research will feature reviews, research articles, brief communications, position papers, letters and patent updates.
Abstract: Reactive oxygen and nitrogen species have been implicated in the onset and progression of various diseases and the role of antioxidants in the maintenance of health and prevention of diseases has received much attention. The action and effect of antioxidants have been studied extensively under different reaction conditions in multiple media. The antioxidant effects are determined by many factors. This review aims to discuss several important issues that should be considered for determination of experimental conditions and interpretation of experimental results in order to understand the beneficial effects and limit of antioxidants against detrimental oxidation of biological molecules. Emphasis was…laid on cell culture experiments and effects of diversity of multiple oxidants on antioxidant efficacy.
Keywords: Antioxidant, cell culture medium, oxygen concentration, reactive oxygen species
Abstract: BACKGROUND: Red raspberry (Rubus idaeus L. ), a natural dietary source of (poly)phenols, has been used as medicine for centuries. OBJECTIVE: The purpose of this study is to determine the effect of a red raspberry (poly)phenolic extract (RPE) on diet-induced obesity, hepatic steatosis and insulin resistance, and elucidate the underlying molecular mechanisms. METHODS: Male specific pathogen-free C57BL/6J mice were randomly divided into three groups (n = 12 per group), and fed with low-fat diet (10% fat energy), high-fat diet (HFD, 45% fat energy), or HFD supplemented with RPE of 150 mg/kg body weight by intragastric administration for 14…weeks. Obesity-related biochemical indexes and hepatic gene expression levels were determined. The statistical analyses were conducted using one-way analysis of variance (ANOVA) followed by Duncan’s multiple range test. RESULTS: The body weight gain, steatosis grade scores and insulin resistance index in the RPE group decreased by 34.48% (P = 0.00), 58.82% (P = 0.00), and 53.77% (P = 0.00), respectively, compared to those in the HFD group. Moreover, RPE supplement significantly changed the expression profile of the genes involved in lipid metabolism and fibroblast growth factor 21 signaling pathway. CONCLUSIONS: This study demonstrated that RPE protected from diet-induced obesity and related metabolic disorders by improving the lipid metabolism and fibroblast growth factor 21 resistance.
Abstract: BACKGROUND: Black raspberry is a vital fruit crop with a high antioxidant function. MADS-box genes play an important role in the regulation of fruit development in angiosperms. OBJECTIVE: To understand the regulatory role of the MADS-box family, a total of 80 MADS-box genes were identified and analyzed. METHODS: The MADS-box genes in the black raspberry genome were analyzed using bioinformatics methods. Through an analysis of the promoter elements, the possible functions of different members of the family were predicted. The spatiotemporal expression patterns of members of the MADS-box family during black raspberry fruit development and ripening…were systematically analyzed. RESULTS: The genes were classified into type I (Mα: 33; Mβ: 6; Mγ: 10) and type II (MIKC * : 2; MIKCC : 29) genes. We also obtained a complete overview of the RoMADS-box gene family through phylogenetic, gene structure, conserved motif, and cis element analyses. The relative expression analysis showed different expression patterns, and most RoMADS-box genes were more highly expressed in fruit than in other tissues of black raspberry. CONCLUSIONS: This finding indicates that the MADS-box gene family is involved in the regulation of fruit ripening processes in black raspberry.
Abstract: BACKGROUND: Black currants are berries that attract attention due to the beneficial effects they possess. They are proven to be good antioxidants, anticancerogenic, cardio- and neuroprotective agents. OBJECTIVE: The main objective of this research was to evaluate the differences in the flavonols composition, antioxidant and spasmolytic activity of juices obtained from four different black currant cultivars – Triton, Tenah, Ben Sarek, and Ometa, grown over a 4-year long period on the territory of Serbia. METHODS: The qualitative and quantitative determination of black currants juices flavonols was performed using the HPLC. The antioxidative activity was carried out…in DPPH and β -carotene systems. The study examined the impact of the main black currants flavonols and black currants juices on the spontaneous, KCl, and acetylcholine-induced ileum smooth muscle contractions. RESULTS: The dominant flavonol in juices of the four different black currant cultivars was myricetin, with its highest content obtained from the 2015 Triton juice (820.1±34.7 μ g g–1 ). Quercetin was the second in abundance and the lowest yields were observed in kaempferol. CONCLUSION: The investigated black currant cultivars were proven to be good sources of flavonols and potential antioxidants with the significant spasmolytic activity that could be successfully used in treating gastrointestinal disorders.
Keywords: Black currants, flavonols, myricetin, spasmolytic activity, juices
Abstract: BACKGROUND: Tomatoes (Solanum lycopersicum ) are important fruits in the Mediterranean diet and are considered to reduce the risk of different human diseases due to their antioxidative powers. OBJECTIVE: The purpose of the present study was to investigate the biodiversity within a set of 50 tomato fruit accessions collected across Turkey concerning their antioxidant capacities and the levels of potential health-beneficial compounds including phenolic compounds, carotenoids, ascorbic acid and tocopherols. METHODS: All accessions were simultaneously grown in an open experimental field in 2017 and ripe fruits were harvested for analysis. Antioxidant capacities of both hydrophilic and…lipophilic extracts were determined using spectrophotometric assays after which individual antioxidants were identified by HPLC using an on-line antioxidant detection system. Phenolic acids, flavonoids, carotenoids and vitamins C and E were quantified using HPLC. RESULTS: The results indicated that there is a wide diversity within this small collection with respect to their hydrophilic and lipophilic antioxidants. CONCLUSION: Hydrophilic antioxidant capacity of the tomatoes was generally related to chlorogenic acid and Vitamin C levels, while lipophilic antioxidants were correlated to all-trans lycopene.
Abstract: BACKGROUND: Polyphenols intake increases the function of brown adipose tissue (BAT), stimulating energy expenditure (EE). Calafate (Berberis microphylla ) is a polyphenol-rich Chilean native fruit. OBJECTIVE: To analyse the effect of a treatment with a Calafate extract in the thermogenic activity of mice adipose tissues. METHODS: Forty adult C57BL/6J male mice were subdivided into four groups (n =10 each): control diet, control+Calafate (extract: 50mg total polyphenols/kg weight), high-fat diet (HF) and HF+Calafate. RESULTS: Calafate prevented the increase in body weight and the decrease EE induced by HF. In BAT, Ucp-1 transcript was influenced by…the interaction between diet and Calafate (p <0.01), Pparα showed the same expression pattern as Ucp-1 and both, diet (p <0.01) and Calafate (p <0.05), induced significant effects in Sirt1. In inguinal adipose tissue, Pgc1α , Pparα , Prdm16, Sirt1, and Dio2 transcripts presented a decreased expression caused by HF, that was reversed by Calafate. In BAT, an effect of diet (p <0.05) and an interaction between diet and Calafate (p <0.01) was observed in UCP-1 protein levels. CONCLUSIONS: A treatment with Calafate drives less weight gain in mice fed with HF, and reverses the effects generated by it on the expression of thermogenic and browning markers.
Keywords: Calafate, polyphenols, brown adipose tissue, thermogenesis, browning
Abstract: BACKGROUND: Monoterpenes and C13 -norisoprenoids are key terpenoid compounds for wine aroma. The enzyme encoded by VviDXS1 participates in terpenoid biosynthesis in grapevine fruits and gain-of-function mutations in this gene lead to characteristic muscat aroma. OBJECTIVE: To assess for VviDXS1 contribution to aroma variation in Northwestern Iberian wine cultivars, we resequenced this gene in 111 cultivars and compared grape juice terpenic composition in 12 of them. METHODS: VviDXS1 was capture-targeted for resequencing with Illumina paired-end reads, SAMtools was used for variant calling and gene haplotypes were reconstructed with PHASE. Monoterpenes and C13 -norisoprenoids…were quantified in free and glycosidically-bound forms from grape juice by gas chromatography-mass spectrometry. RESULTS: Terpenic composition discriminated between muscat, terpenic and neutral profiles across cultivars. While the terpenic profile of Loureira and Albariño white cultivars was not associated with muscat-like mutations, Albariño carries a V34L substitution in VviDXS1 that is also present in other aromatic cultivars and was not reported before. Tempranillo and Cabernet Sauvignon red cultivars accumulated higher levels of C13 -norisoprenoids, which was not associated with specific variation in VviDXS1 . CONCLUSIONS: Apart from the uncharacterized substitution present in Albariño, findings suggest that terpenoid pathway-related genes other than VviDXS1 could contribute to the aromatic attributes of these cultivars.
Keywords: DXS, haplotype diversity, monoterpenes, C13-norisoprenoids, muscat aroma
Abstract: BACKGROUND: HD-Zip genes encode several large and highly conserved protein families of transcription factors that play important roles in plant development and responses to environmental stress. To date, information about the involvement of HD-Zip in grape to drought response is limited. OBJECTIVE: The production of grapes is limited by a range of biotic and abiotic stresses, which cause significant losses in yield every year as well as a reduction in fruit quality. Identification and analysis of stress related genes in grapes are very important for cultivating more robust varieties with environmental stress resistance METHODS: We isolated…a homeodomain-leucine zipper gene (HD-Zip), VvHDZ4 , from grape, Vitis vinifera L. “Yatomi Rose”, and characterized VvHDZ4 based on analyses of its expression patterns, subcellular localization, transcription activity, and overexpression. RESULTS: We found that VvHDZ4 was highly expressed in roots and leaves, localized to nucleus, and activated the expression of reporter genes in yeast. The overexpression (OE) of VvHDZ4 led to enhanced drought stress tolerance in Solanum lycopersicum L. “Ailsa Craig” (tomato). The drought-tolerant phenotypes of the OE lines exhibited decreases in electrolyte leakage and rate of water loss, higher photosynthetic production and net photosynthesis rate, and showed up-regulation of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX), all of which are key antioxidant enzymes known to be activated during drought stress. Moreover, we observed the up-regulation of four additional drought stress indicator genes, SIDREB2A , SIAREB , SIRD29 and SIERD10 , in the OE lines. VvHDZ4 improved drought tolerance in tomato, and the modes of action possibly consist of reducing membrane damage, increasing photosynthetic productivity, and modulating the expression of stress defense genes. CONCLUSIONS: This work increases our understanding of the important roles HD-Zip transcription factors in the responses of plants to the environment, especially abiotic stress.
Abstract: BACKGROUND: Fruits of Crataegus songarica are commonly used for the treatment of vascular insufficiency and heart problems. OBJECTIVE: Our aim was to determine the effect of C. songarica on vascular tone and to determine the mechanisms underlying the vasorelaxant properties. METHODS: Extracts of C. songarica were tested for vasodilator activity of porcine coronary artery after pre-contraction with the thromboxane mimetic U46619 in the presence or absence of inhibitors of intracellular signaling cascades. Reactive oxygen species were assessed by dihydroethidine staining and the level of eNOS and AKT phosphorylation was measured by immunohistochemical staining.…RESULTS: Extracts of C. songarica berries produced endothelium dependent vasorelaxation, with most significant effect induced by aqueous fraction (AS-CS). This vasorelaxant effect of AS-CS was reduced by inhibition of nitric oxide pathways and inhibition of potassium channels. Inhibition of phosphatidylinositol 3- kinase and Src tyrosine kinase, as well as scavenging of reactive oxygen species, produced an attenuation of the relaxation response. Estrogen receptor antagonists (tamoxifen and ICI 182,782) reduced the AS-CS mediated vasorelaxation. AS-CS also stimulated the endothelial formation of ROS and phosphorylation of Akt and eNOS. CONCLUSION: The data indicated that C. songarica produces an endothelium-dependent vasorelaxation, which is partly dependent upon estrogen receptors, and sensitive to inhibition of ROS/Src/PI3K/NO pathways.
Abstract: BACKGROUND: In the Mediterranean basin, the solar radiation received is very high for many tree species over several hours of the day during the summer. Using a photoselective net can improve the climatic conditions and also modify the quality of light received. OBJECTIVE: This study aimed to evaluate the influence of the colour of photoselective nets on the yield and quality of Actinidia chinensis . MATERIALS: The experiment was carried out in Southern Italy on an orchard of Jintao kiwifruit. Photoselective nets of different colours were used: black, red, yellow, white, and grey. The resulting fruits…were compared to those of plants in the open field. A randomised block design was adopted. Environmental, vegetative, and leaf gas exchange parameters; yield; and fruit quality were defined. All data were analysed using the Variance Analysis (ANOVA) and the Principal Component Analysis (PCA). RESULTS: Photoselectivity influenced production. The best results were obtained under the red net in terms of production and fertility indices. Fruit size, maturation indices, and nutraceutical parameters were also higher under the red net. Among the nets, the grey net induced the worst tree productivity results and the worst fruit quality parameters. However, all nets showed better results compared to the open field. CONCLUSION: The photoselective net strongly influenced the yield and quality of Jintao kiwifruit and the better results compared to the open field. The red net proved to be the best performer for the environment where the experiment was carried out.
, maturation indices, photoselective net