Cancer Biomarkers - Volume 30, issue 2

Authors: Hussein, Samia | Fathi, Anan | Abouhashem, Nehal S. | Amer, Samar | Hemeda, Mohamed | Mosaad, Hala

Article Type: Research Article

Abstract: Studying bladder cancer molecular biology revealed the presence of genetic alterations. So, detection of molecular biomarkers that help in monitoring the disease, evaluating the prognosis of the patients, and their response to therapy is needed. In this study, we investigated the expression and the prognostic significance of SATB-1 and ERBB2 mRNA and protein by quantitative RT-PCR and immunohistochemical analysis in urothelial bladder cancer cases and the surrounding normal bladder tissue. The correlations between the expression of both markers and the clinicopathological parameters were performed with further analysis of the correlation between the expression of SATB-1 and ERBB2. Compared to control, …the expression of SATB-1 and ERBB2 mRNA and protein in cancer tissues were significantly up-regulated (p < 0.05). Also, a positive correlation between both markers was found (r = 0.53, p < 0.001). Moreover, elevated levels of both markers were significantly associated with the stage, lymph node involvement at both mRNA and protein levels (p < 0.001). In conclusion, there is a clinical significance of SATB-1 and ERBB2 as potential biomarkers for predicting bladder cancer patients of aggressive behavior and poor prognosis. Show more

Keywords: RT-PCR, immunohistochemistry, bladder cancer, SATB-1, ERBB2

DOI: 10.3233/CBM-200072

Citation: Cancer Biomarkers, vol. 30, no. 2, pp. 249-259, 2021

Authors: Zhong, Youqing | Lin, Hui | Li, Qi | Liu, Chang | Shen, Jinmei

Article Type: Research Article

Abstract: Circular RNAs (circRNAs) have been revealed to involve in the chemoresistance of various cancers, including non-small cell lung cancer (NSCLC). Here, we further investigate the role of circRNA_100565 in NSCLC cisplatin (DDP) resistance. The expression of circRNA_100565 and microRNA (miR)-337-3p, and ADAM metallopeptidase domain 28 (ADAM28) mRNA was detected using quantitative real-time polymerase chain reaction. Cell viability and apoptosis were measured by cell counting kit-8 assay and flow cytometry, respectively. Western blot was used to detect the level of ADAM28 and autophagy-related protein. The interaction between miR-337-3p and circRNA_100565 or ADAM28 was confirmed by dual-luciferase reporter assay or pull-down assay. …In vivo experiments were conducted via the murine xenograft model. We found CircRNA_100565 was up-regulated in NSCLC DDP-resistant tissues and cell lines, and its high expression was associated with shorter overall survival of NSCLC patients. CircRNA_100565 deletion mitigated DDP resistance, reflected by the suppression of proliferation and autophagy, the reduction of IC50 value, as well as enhancement of apoptosis in DDP-resistant NSCLC cells. MiR-377-3p was confirmed to directly bind to circRNA_100565 or ADAM28 3’-UTR. Moreover, circRNA_100565 indirectly regulated ADAM28 expression by sponging miR-377-3p in NSCLC cells. Additionally, circRNA_100565 deletion-induced sensitivity of NSCLC resistant cells to DDP could be remarkably attenuated by miR-377-3p inhibition or ADAM28 re-expression. Meanwhile, circRNA_100565 knockdown contributed to the anti-tumor effects of DDP on NSCLC in vivo . CONCLUSION: CircRNA_100565 was an independent prognostic factor for NSCLC patient survival, and enhanced the resistance of NSCLC cells to cisplatin by regulating cell proliferation, apoptosis and autophagy via miR-337-3p/ADAM28 axis, shedding light on the development of a novel therapeutic strategy to boost the effectiveness of NSCLC chemotherapy. Show more

Keywords: circRNA_100565, miR-337-3p, ADAM28, NSCLC, cisplatin resistance

DOI: 10.3233/CBM-201705

Citation: Cancer Biomarkers, vol. 30, no. 2, pp. 261-273, 2021

Article Type: Correction

DOI: 10.3233/CBM-210950

Citation: Cancer Biomarkers, vol. 30, no. 2, pp. 275-, 2021