Cancer Biomarkers - Volume 23, issue 1

Authors: Cheng, Jin-Zhang | Chen, Jun-Jun | Wang, Zong-Gui | Yu, Dan

Article Type: Research Article

Abstract: This article has been retracted, and the online PDF replaced with this retraction notice. doi: 10.3233/CBM-220951

Keywords: microRNA-185, HOXC6, TGF-β1/mTOR axis, nasopharyngeal carcinoma, proliferation, apoptosis, autophagy

DOI: 10.3233/CBM-181459

Citation: Cancer Biomarkers, vol. 23, no. 1, pp. 107-123, 2018

Authors: Baranova, Ivana | Kovarikova, Helena | Laco, Jan | Dvorak, Ondrej | Sedlakova, Iva | Palicka, Vladimir | Chmelarova, Marcela

Article Type: Research Article

Abstract: BACKGROUND: Aberrant DNA methylation of protocadherins (PCDHs) has been associated with development and progression of various types of cancer. It could represent possible direction in the search for critically needed tumor biomarkers for ovarian cancer. OBJECTIVE: To investigate methylation of δ 2 group of non-clustered PCDHs in high-grade serous ovarian carcinoma (HGSOC) tissue in comparison with control tissue. METHODS: We used next-generation sequencing for detecting regions with the most altered methylation. For further confirmation of discovered alterations we used methylation-sensitive high-resolution melting analysis. RESULTS: PCDH17 methylation was detected …in almost 70% of HGSOC patients without any methylation in the group of control samples and was found both in the late stage tumors as well as in the early stage ones. Other selected PCDHs did not show any relevant changes in methylation. Subsequent gene expression analysis of PCDH17 revealed decreased expression in all of the tumor samples in comparison to the control ones. Statistically significant negative correlation was found between methylation and levels of expression suggesting potentially methylation-based silencing. CONCLUSIONS: Methylation of PCDH17 could play an important role in development and progression of HGSOC and has potential to become a target in the search for new clinical biomarkers. Show more

Keywords: Ovary, high-grade serous carcinoma, methylation, protocadherin, next-generation sequencing, biomarker

DOI: 10.3233/CBM-181493

Citation: Cancer Biomarkers, vol. 23, no. 1, pp. 125-133, 2018

Authors: Kim, Yi Rang | Byun, Mi Ran | Choi, Jin Woo

Article Type: Research Article

Abstract: BACKGROUND: Hepatitis B virus (HBV) accounts for more than 60% of hepatocellular carcinoma (HCC) cases. However, there is limited information about the features of HBV-driven HCC that differentiate it from other types of HCC. OBJECTIVE: The aim of this study is to find a gene specific to HBV-driven HCC and understand its role during tumorigenesis. METHODS: The differences in gene expression patterns were analyzed among patients with hepatitis virus-unrelated liver cirrhosis, and hepatitis C virus- and HBV-driven HCC. Genes expressed only in HBV patients were compared to genes of transgenic mice expressing hepatitis …B viral X gene. RESULTS: Integrin α 6 was commonly overexpressed in both HBV-driven HCC patients and transgenic mice expressing viral X. This gene’s activation induced overexpression of integrin α 6, as well as formation of integrins α 6β 1 and α 6β 4, without changing the expression of non-integrin laminin receptors. Suppression of integrin α 6 caused significant inhibition of tumor migration in vitro . CONCLUSIONS: This study found a significant association between HBV and integrin α 6, which may be responsible for early migration and invasion of HCC. Thus, integrin α 6 is a predictive marker for tumor recurrence and invasiveness of HBV-driven HCC. Show more

Keywords: Hepatocellular carcinoma, integrin, hepatitis B virus, hepatitis B viral X gene

DOI: 10.3233/CBM-181498

Citation: Cancer Biomarkers, vol. 23, no. 1, pp. 135-144, 2018

Authors: Cai, Yu | Yan, Pu | Zhang, Ge | Yang, Wenqi | Wang, Haiping | Cheng, Xiaohu

Article Type: Research Article

Abstract: OBJECTIVE: Colorectal cancer (CRC) is the 3 rd most common cancer worldwide. Recently, long non-coding RNAs (lncRNAs) were found to be critical modulators in the CRC progression. The aim of this study is to investigate the potential roles of lncRNA P73 antisense RNA 1T (TP73-AS1) in CRC development and progression. METHODS: Quantitative real-time PCR (qRT-PCR) was performed to determine relevant gene expression levels; western blot was performed to determine protein expression levels; CCK-8, colony formation, wound healing and Transwell invasion assays were used to determined CRC cell proliferation, migration and invasion; in …vivo tumor growth was assessed in xenograft mice model. RESULTS: TP73-AS1 was up-regulated in both CRC tissues and CRC cell lines. Overexpression of TP73-AS1 was associated with metastasis and advanced clinical stages in CRC patients. Overexpression of TP73-AS1 promoted CRC cell growth, proliferation, migration and invasion in vitro ; and knockdown of TP73-AS1 significantly inhibited CRC cell growth, proliferation, migration and invasion in vitro as well as tumor growth in vivo . Bioinformatics analysis and luciferase reporter assay indicated that TP73-AS1 could bind directly with miR-194, and TP73-AS1 negatively regulated the expression of miR-194 in CRC cells. Further study indicated that miR-194 negatively regulated the downstream target of transforming growth factor alpha (TGFα ) via targeting its 3’ untranslated region, and TP73-AS1 positively regulated the expression of TGFα in CRC cells. Moreover, overexpression of miR-194 suppressed CRC cell proliferation and invasion, and attenuated the effects of TP73-AS1 overexpression on CRC cell proliferation and invasion. Silence of TGFα inhibited CRC cell proliferation and invasion, and also reversed the effects of TP73-AS1 overexpression on CRC cell proliferation and invasion. CONCLUSIONS: this study demonstrated that TP73-AS1 regulated CRC progression by acting as a competitive endogenous RNA to sponge miR-194 to modulate the expression of TGFα . Show more

Keywords: Colorectal cancer, progression, TP73-AS1, miR-194, TGFα

DOI: 10.3233/CBM-181503

Citation: Cancer Biomarkers, vol. 23, no. 1, pp. 145-156, 2018