Affiliations: [a] Department of Molecular Biology and Genetics, Yıldız Technical University, Istanbul, 34349, Turkey | [b] Medical Biology Department, Faculty of Medicine, Kırşehir Ahi Evran University, Kırşehir, 40100, Turkey | [c] Advanced Technologies Research and Application Center, Hacettepe University, Ankara, 06800, Turkey | [d] Department of Chemistry, Polatlı Faculty of Arts and Sciences, Ankara Hacı Bayram Veli University, Ankara, 06900, Turkey | [e] Department of Orthopedics and Traumatology, Yenimahalle Training and Research Hospital, Ankara, 06800, Turkey
Correspondence:
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Corresponding author: Murat Demirbilek, Advanced Technologies Research and Application Center, Hacettepe University, Ankara, 06800, Turkey. Tel.: +90 5063978019; E-mail: [email protected]@gmail.com.
Abstract: BACKGROUND: Bioengineering products can help bone tissue regeneration. OBJECTIVE: There is an ongoing research for more effective biomaterials in bone regeneration. Chitosan (Ch) grafted stearic acid (Ch-g-Sa) polymer was synthesized and its usability as a putty was evaluated in this study. METHODS: The chemical structure of Ch-g-Sa polymer was investigated using Proton nuclear magnetic resonance (H-NMR) and Fourier-transformed infrared spectroscopy-attenuated total reflectance (FTIR-ATR). Thermal properties of Ch-g-Sa polymer were determined by thermal gravimetric analysis (TGA). Putties containing nano-hydroxyapatite were prepared and in-vitro degradation properties and viscosity of the putties were determined. RESULTS: The cytotoxicity, oxidation effect and osteogenic potential of the putties were investigated on MC3T3 cells while the inflammatory effect of the putties was studied on THP-1 cells. For the determination of the osteogenic effect of the putties, ALP and RUNX2 gene expression of MC3T3 cells were studied. CONCLUSION: Ch-g-Sa/HA putties are promising biomaterials for bone tissue regeneration.
