Affiliations: [a] Department of Computer Engineering, Hallym University, Chuncheon, Korea | [b] Department of Convergence Software, Hallym University, Chuncheon, Korea | [c] Bio-IT Research Center, Hallym University, Chuncheon, Korea
Correspondence:
[*]
Corresponding author: Chan-Young Park, Department of Convergence Software, Hallym University, Chuncheon, Korea. E-mail:[email protected]
Abstract: This paper proposes the optimal structure of a PCB-based
micro PCR chip constructed on a PCB substrate using commercial adhesive
tapes and plastic covers. The solder mask of the PCB substrate was coated
black, and the area where the reaction chamber is attached was legend
printed with white silk to minimize the noise during fluorescence detection.
The performance of the PCR and fluorescence detection was compared using 6
types of reaction chambers, each made with different double-sided tapes.
Three of the chambers were unsuccessful in completing the PCR. The
performance of the other three chambers that successfully amplified DNA was
compared using Taqman probe for Chlamydia Trachomatis DNA. The amplified
product was illuminated diagonally with a blue LED to excite the product
just before imaging, and the LED was turned off when the image was captured
to prevent quenching of the probe. The images were taken 10 seconds prior to
the last extension step for each cycle using a DSLR camera. The experiments
were run as a quartet for each three chambers made with different
double-sided tape. The results showed that there were significant difference
between the three tapes.
