2nd Dimensional GC-MS analysis of sweat volatile organic compounds prepared by solid phase micro-extraction

Issue title: Recent Trends in Convergence-based Smart Healthcare Service

Guest editors: Sang-Yeob Ohv, Supratip Ghosew, Kyungyong Chungx, Joong-Kyung Ryuy and Jung-Soo Hanz

Article type: Research Article

Authors: Choi, Mi-Jung^a | Oh, Chang-Hwan^{b; *}

Affiliations: [a] Department of Crime Scene Investigation, Hyechon College, Daejeon, Korea | [b] Department of Oriental Medical Food and Nutrition, Semyung University, Chungbuk, Korea | [v] Department of Interactive Media, Gachon University, Seongnam, Korea | [w] Department of Computer Science and Engineering, University of Information Technology and Sciences, Dhaka, Bangladesh | [x] Department of Computer Information Engineering, Sangji University, Wonju, Korea | [y] Department of Computer Software, Daelim University, Gyeonggi-do, Korea | [z] Division of Information and Communication, Baekseok University, Seoul, Korea

Correspondence: [*] Corresponding author: Chang-Hwan Oh, Department of Oriental Medical Food and Nutrition, Semyung University, 65 Semyung-ro, Jecheon, Chungbuk 390-711, Korea. E-mail: [email protected].

Abstract: The characteristics of an individual's odor from sweat, breath and skin provide important information for criminal tracking in field of forensic science. Solid phase micro-extraction gas chromatography/mass spectrometry (SPME-GC/MS) was used to determine human sweat volatile organic compounds (VOCs) profiles. The mass spectrometric analysis (with electron impact mode) followed by 2nd dimensional separation with two different GC columns (one polar and one relatively nonpolar) connected in parallel were used to identify the 574 compounds from sweat samples. The components included alcohols, aldehydes, aliphatics/aromatics, carboxylic acids, esters, ketones, and other organic compounds (amides/amines, thio/thioesters, oxide, sulfides, nitro compounds). Of these compounds, 1-tridecanol, 1,3-bis(1,1-dimethyl ethyl)-benzene, 4,4'-(1-methylethylidene) bis-phenol and 7-acetyl-6-ethyl-1,1,4,4,-tetramethyl-tetraline were common components in all donor's sweat volatile samples. Age-related specific compounds were also detected. The results suggest that characteristic volatile profiles of human sweat emanations could provide the valuable information to forensic scientists.

Keywords: GC × GC-TOFMS, sweat, volatile organic compounds (VOCs), solid phase micro-extraction (SPME)

DOI: 10.3233/THC-140807

Journal: Technology and Health Care, vol. 22, no. 3, pp. 481-488, 2014