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Article type: Research Article
Authors: Haasper, Carla; * | Breitbart, Astrida | Hankemeier, Stefana | Wehmeier, Michaelb | Hesse, Erica | Citak, Musaa | Krettek, Christiana | Zeichen, Johannesa | Jagodzinski, Michaela
Affiliations: [a] Trauma Department, Hannover Medical School (MHH), Hannover, Germany | [b] Department of Clinical Chemistry, Hannover Medical School (MHH), Hannover, Germany
Correspondence: [*] Address for correspondence: Dr. Carl Haasper, Trauma Department, Hannover Medical School (MHH), Carl-Neuberg-Str. 1/OE 6230, D-30625 Hannover, Germany. Tel.: +49 511 532 2050; Fax: +49 511 532 5877; E-mail: [email protected].
Abstract: For tissue engineering of bone, a carrier matrix and efficient cell seeding are desirable. This study analysed the effect of fibrin glue on bone marrow stromal cells (BMSC) adhesion, proliferation (MTS-Test), differentiation (alkaline phosphatase (AP), osteocalcin (OC), ELISA) and compared the results with cells seeded within culture media on a decellularized, xenogenic bone matrix. There was no significant difference regarding cell adhesion. Proliferation after one week was significantly increased without fibrin glue. AP was increased in both groups when compared with porous scaffolds without cells. OC secretion was increased under both seeding conditions. Microscopic investigation of the cells with fibrin-glue showed less cell-cell contacts. This study reveals that cell seeding with medium demonstrates similar adherence rates compared with fibrin glue. Fibrin glue significantly decreases cell proliferation. Cell differentiation with respect to ALP and OC is not affected. Further studies are required to assess the long term and in vivo effects of both methods with respect to BMSC viability and differentiation. Fibrin sealants seem not necessary to achieve cell adherence when using a porous bone matrix.
Keywords: Tissue engineering, bone, fibrin glue, cell culture
DOI: 10.3233/THC-2008-16203
Journal: Technology and Health Care, vol. 16, no. 2, pp. 93-101, 2008
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