Abstract: OBJECTIVE: This study examined the association between body mass index (BMI), physical activity and cardiorespiratory fitness levels on work performance with and without neck pain in habitual computer users. METHODS: Forty participants with and without neck pain were recruited and completed a battery of measures: body composition (BMI), physical activity levels (International Physical Activity Questionnaire), cardiorespiratory fitness (Chester Step Test) and work performance (Health and Work Performance Questionnaire). MAIN RESULTS: Body mass index, physical fitness and cardiorespiratory fitness were not significantly associated with the three domains of workplace performance; absenteeism, presenteeism and critical incidents. However, a significant association was found between neck disability and the incidence of headaches/migraines and fatigue/sleep disturbance. CONCLUSION: Mild neck pain in habitual computer users does not significantly influence work performance, although the association between the co-morbidities (headaches/migraine and fatigue/sleep disturbance) should be considered in those presenting for treatment with mild neck pain.