Affiliations: [a] Department of Nutrition, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran. e-mail: [email protected]
| [b] Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran | [c] Division of Biochemistry, Department of Chemistry, Pune University, Pune 411007, India. e-mail: [email protected]
F. Montazerifar and M. Dikshit share senior authorship.
Abstract: The aim of this study was to evaluate the antioxidant supplementation effect on antioxidant system in hemodialysis patients. The study group consisted of 38 patients undergoing hemodialysis and 72 healthy subjects. The patients orally received combination of vitamins C (250 mg/time) and E (200 IU/time) three times per week for 2 months. Both, enzymatic antioxidants [superoxide dismutase (SOD) and glutathione peroxidase (GPx)] activities and non-enzymatic (vitamins E and C) levels and malondialdehyde (MDA) concentrations as lipid peroxidation product in patients were measured before and after supplementation compared with control group. The levels of antioxidants (vitamin E, C and SOD) (P < 0.0001) and GPx (P < 0.05) were significantly enhanced and MDA concentrations declined (P < 0.0001) during the supplementation period. The study demonstrates that combined supplementation of vitamins C and E can noticeably affect antioxidant status and protect against oxidative stress caused during hemodialysis.
Keywords: Hemodialysis, Combined supplementation, Vitamin C, Vitamin E, Oxidative stress