Affiliations: [a] Department of Biochemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh 517502, India. e-mail: [email protected]
Abstract: Glutathione S-transferases (GSTs, EC 184.108.40.206), as the detoxifying enzymes, play an important protective role in embryonic tissues. The GSTs of eleventh-day chick embryonic liver were purified to electrophoretic homogeneity with an overall yield of 31%. The SDS-PAGE analysis of chick embryonic liver GSTs resolved in to three bands, CL1, CL2 and CL3, with relative molecular weights of 27.0, 26.0 and 25.0 kDa respectively. On 72 hours of treatment with acrylamide (AC) (0.1, 0.2 and 0.3 mg) the specific activities of GSTs with CDNB, pNBC, EPNP, BSP, Δ5A and pNPA increased significantly. Total GPx levels were decreased with CHP in liver samples. Immunoblot analysis of AC-treated liver samples showed a dose-dependent increase in the induction of GSTs. CL1 of theta class, CL2 of Mu class and CL3 of alpha class were induced with AC treatment. The theta, mu and alpha classes induced by AC might inhibit the initiation of carcinogenesis.
Keywords: Glutathione S-transferases, Acrylamide, Substrate specificities, Chick embryonic liver, Western blot