Affiliations: [a] Bioconversion, Microbiology Engineering and Health Safety Laboratory (LBGMSS), Nature and Life Sciences Faculty, Mustapha Stambouli University, Mascara, Algeria
| [b] Department of Basic Medical Sciences, Faculty of Medicine, Yarmouk University, Irbid, Jordan
| [c] Pediatric Neonatal Department, CHU Nord-Amiens, France
Corresponding author: Foufa Bouabsa, Ph.D student, Bioconversion, Microbiology Engineering and Health Safety Laboratory (LBGMSS), Nature and Life Sciences Faculty, Mustapha Stambouli University, Mascara, 29000, Algeria. E-mail: [email protected].
Abstract: BACKGROUND:Caffeine is a potent central and respiratory acting agent used in neonatology to treat apnea in premature newborns. OBJECTIVE:This study investigates the effects of caffeine orally administered to newborn rats on gastrointestinal permeability, bacterial translocation and different biochemical parameters. METHODS:Newborn rats were divided into different groups (N = 06). The treated newborn rats were orally administered with standard caffeine doses (12 mg/kg per day), and the control groups received a placebo. The animals were weighed daily until sacrifice. Blood samples, mesenteric lymph nodes (MLN) and organs were aseptically collected. Furthermore, different biochemical (D-Lactate) and oxidative stress biomarkers (MDA, CAT, SOD and GSH) were examined. Microbiological analyses were performed to assess microbiota alterations and bacterial translocation. RESULTS:Preliminary results showed that caffeine administration decreased the level of bacterial translocation over time. The treatment reduced plasma D-lactate levels (p < 0.05). Additionally, caffeine induced a disturbance in the concentrations of biochemical parameters and oxidative stress biomarkers. Indeed, liver enzymes (AST and ALT) were significantly (p < 0.05) risen after caffeine treatment. Glutathione (GSH) levels were significantly higher in caffeine treated groups (75.12±0.32; 51.98±1.12 U/mg; p < 0.05) comparing to control ones (40.82±0.25; 42.91±0.27 U/mg; p < 0.05) in the ileum and the colon, respectively. CONCLUSIONS:Thus, besides improving gastrointestinal permeability, our data show that caffeine has beneficial effects on the intestinal antioxidant system.