Department of Clinical Biochemistry, Hamadan University of Medical Sciences, Hamadan, Iran
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Nutrition Health Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
Corresponding author: Heidar Tavilani, Department of Clinical Biochemistry, Hamadan University of Medical
Sciences, Hamadan, Iran. Shaheed Fahmideh Ave., Hamadan, Iran. Tel.: +98 8138381590; E-mails: [email protected] and [email protected].
Abstract: BACKGROUND:Deleterious consequences of a high- fat diet have been established. OBJECTIVE:The aim of the present study was to investigate the effects of kiwifruit (as a rich source of antioxidants) on the activity and gene expression of NADPH oxidase in high- fat diet (HFD) fed hamsters. METHODS:Thirty male Syrian hamsters were divided into six groups and fed control diet, control diet with kiwifruit (1.86 or 3.73 g/kg), or HFD (15% butter fat 0.05% cholesterol) with or without kiwifruit (1.86 g/kg or 3.73 g/kg) for eight weeks. RESULTS:The results revealed that gene expression levels of gp91phox and p47phox, activity of NADPH oxidase, and level of TBARS were significantly increased in the liver of high fat diet group compared with control normal group (p < 0.001). NADPH Oxidase activity and gene expression levels of gp91phox and p47phox were significantly decreased in groups fed high- fat diet supplemented with kiwifruit (p < 0.05). Moreover, in comparison with control high-fat diet group, a significant decrease was observed in TBARS levels of high- fat diet supplemented with 3.73 g/kg kiwifruit (p < 0.001). CONCLUSIONS:The consumption of kiwifruit in high-fat diet fed hamsters may improve hepatic oxidative stress by preventing lipid peroxidation and reducing the activity and gene expression of NOX2.