Affiliations: [a] Department of Pharmaceutical Sciences, North South University, Bangladesh
| [b] BCSIR Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Bangladesh
Corresponding author: Dr. Muhammad Ashrful Alam, Department of Pharmaceutical Sciences, North South University, Bangladesh. E-mail: [email protected].
Note:  Equal contribution.
Abstract: Beetroot (Beta vulgaris) has been reported to possess many benefits and medicinal properties. However, the protective effect of beetroot against isoproterenol (ISO) induced myocardial damage has not been clarified sufficiently yet. The present study was aimed to investigate the effects of beetroot on oxidative stress, fibrosis, and myocardial damage in ISO-induced rats. Our investigation revealed that ISO administration markedly increased the oxidative stress parameters, while the level of cellular antioxidants and catalase activities were decreased in ISO-administered rats. Beetroot supplementation and gallic acid treatment to ISO-administered rats prevented the rises of lipid peroxidation products malondialdehyde, nitric oxide and advanced protein oxidation product concentration. Moreover, elevated activities of alanine aminotransferase, aspartate aminotransferase, and creatinine kinase-muscle brain enzymes activities in ISO-administered rats were also lowered by both gallic acid and beetroot supplementation. Furthermore, gallic acid and beetroot supplementation prevented the inflammatory cells infiltration and fibrosis in ISO-induced rats. In conclusion, these results suggest that beetroot supplementation is capable of protecting ISO-administered myocardial infarction in rats probably by preventing oxidative stress.