Functional Connectivity Analysis in Heterozygous Glucocerebrosidase Mutation Carriers

Article type: Research Article

Authors: Sezgin, Mine^{a; *} | Kicik, Ani^{b; c} | Bilgic, Basar^a | Kurt, Elif^{b; d} | Bayram, Ali^{b; d} | Hanagası, Hasmet^a | Tepgec, Fatih^e | Toksoy, Guven^e | Gurvit, Hakan^a | Uyguner, Oya^e | Gokcay, Gulden^f | Demiralp, Tamer^{b; g} | Emre, Murat^a

Affiliations: [a] Istanbul University, Istanbul Faculty of Medicine, Department of Neurology, Istanbul, Turkey | [b] Istanbul University, Hulusi Behcet Life Sciences Research Laboratory, Istanbul, Turkey | [c] Istanbul Bilim University, Faculty of Medicine, Department of Physiology, Istanbul, Turkey | [d] Department of Neuroscience, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey | [e] Istanbul University, Istanbul Faculty of Medicine, Department of Medical Genetics, Istanbul, Turkey | [f] Istanbul University, Istanbul Medical Faculty, Department of Pediatrics, Division of Pediatric Nutrition and Metabolism, Istanbul, Turkey | [g] Istanbul University, Istanbul Faculty of Medicine, Department of Physiology, Istanbul, Turkey

Correspondence: [*] Correspondence to: Mine Sezgin, MD, Istanbul University, Istanbul Faculty of Medicine, Department of Neurology, 34390 Capa, Istanbul, Turkey. Tel.: +90 212 414 20 00; E-mail: [email protected].

Abstract: Background:There is evidence that alterations in functional connectivity (FC) of the striatocortical circuits may appear before the onset of clinical symptoms of Parkinson’s disease (PD). Objective:The aim of this study was to investigate FC of the striatocortical circuitry in asymptomatic carriers of heterozygous glucocerebrosidase (GBA) mutations, which pose a significant risk for developing PD. Methods:Twenty-one parents of confirmed Gaucher disease patients who were carrying heterozygous GBA mutations and 18 healthy individuals matched for age and gender were included. GBA mutation analysis was performed in all participants. Clinical evaluation included neurological examination, Mini Mental State Examination, and UPDRS Part III. Structural and functional MRI data of 18 asymptomatic GBA mutation carriers (asGBAmc) and 17 healthy controls (HC) were available. FC was analyzed with seed-based approach. Results:Eleven asymptomatic mutation carriers had heterozygous p.L483P mutation, 6 subjects heterozygous p.N409S mutation and 1 subject heterozygous p.R392G mutation in GBA gene. Mini-Mental State Examination mean score was 28.77 (±1.16) and 29.64 (±0.70) in asGBAmc and HC groups, respectively (p = 0.012). Significant increased connectivity Conclusion:Our results suggest that alterations in striatocortical FC can be detected in asymptomatic heterozygous GBA mutation carriers who are at risk of developing PD. These findings may provide insight into network changes during the asymptomatic phase of PD.

Keywords: Parkinson’s disease, glucoserobrosidase, GBA, functional connectivity, resting state fMRI

DOI: 10.3233/JPD-202295

Journal: Journal of Parkinson's Disease, vol. Pre-press, no. Pre-press, pp. 1-10, 2021