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Evolvability and Neurodegenerative Disease: Antagonistic Pleiotropy Phenomena Derived from Amyloid Aggregates

Abstract

At present, the precise physiological role of neurodegenerative disease-related amyloidogenic proteins (APs), including α-synuclein in Parkinson’s disease and β-amyloid in Alzheimer’s disease, remains unclear. Because of similar adaptability of both human brain neurons and yeast cells to diverse environmental stressors, we previously proposed that the concept of evolvability in yeast prion could also be applied to APs in human brain. However, the mechanistic relevance of evolvability to neurodegenerative disorders is elusive. Therefore, our objective is to discuss our hypothesis that evolvability and neurodegenerative disease may represent a form of antagonistic pleiotropy derived from the aggregates of APs. Importantly, such a perspective may provide an outlook of the entire course of sporadic neurodegenerative diseases.

The physiological functions of the amyloidogenic proteins (APs) from neurodegenerative diseases, such as α-synuclein (αS) in Parkinson’s disease (PD) and β-amyloid (Aβ) in Alzheimer’s disease (AD), is currently unknown [1]. In this regard, many of the previous studies have described that APs might be involved in the vesicle functions. For instance, αS was shown to regulate the size of the presynaptic vesicle pools in primary hippocampal neurons [2] and to promote SNARE-complex assembly [3]. Similarly, Aβ has been characterized as a modulator of synaptic plasticity, which may be important in the regulation of learning and memory [4, 5]. These findings are consistent with a notion that impairment of APs may underlie the pathogenesis of neurodegenerative diseases, but may not explain why APs are expressed ubiquitously in neuronal and non-neuronal tissues.

Evolvability of yeast prion represents an amyloid-based genetic switch that confers multiple phenotypes for yeast to cope with diverse stressors in the fluctuating environment [6]. Given that the human brain is exposed to multiple stressors, including hyperthermia, oxidative stress, kindling, physical stress and neurotoxicity, a similar concept of the evolvability could be applied to APs, including αS and Aβ [1]. Briefly, the heterogeneity of the stress-induced aggregates of APs might confer a phenotypic diversification that may be adaptive against such diverse stresses, in essence a form of hormesis [1]. Subsequently, stress-induced APs might be transmitted from parents to offspring via the germ line, leading to a preconditioning of offspring [1]. In support of our hypothetical view, recent studies reveal that APs are composed of structurally distinct populations in neurodegenerative conditions such as PD and AD [7, 8]. Furthermore, accumulating evidence suggests that the transgenerational transmission that enables inheritance of the acquired characteristics to environmental stresses not only occurs in microorganisms but also in mammals [9, 10]. Moreover, it was recently shown that semen contained multiple types of amyloid fibrils in the absence of disease, although their biological roles are unknown [11]. Taken together, it is suggested that APs in human brain may also demonstrate evolvability, which is evolutionarily advantageous for the neuronal survival of offspring, although the transgenerational transmission of APs is still elusive.

In contrast to the obvious benefits of APs evolvability during reproductive stages, it has been well established that APs aggregation may play a central role in the pathogenesis of neurodegenerative disorders in aging [12]. Indeed, a number of studies have shown that prefibrillar aggregates, including oligomers and misfolded monomers, may be more neurotoxic than mature fibrils [13]. Thus, the processes of evolvability and neurodegeneration may both be attributable to APs aggregation, and furthermore, it is possible that the detrimental effects of APs aggregation in neurodegenerative disorders may not have been weeded out because of the biological benefits of evolvability in the earlier reproductive stage (Fig. 1). This is reminiscent of the antagonistic pleiotropy hypothesis proposed by G. C. Williams a half century ago, suggesting that certain genes whose functions are beneficial during reproductive stages may exert later adverse effects in aging (Fig. 1) [14]. Although there had been few clear-cut examples of candidate genes [15], a recent study revealed pleiotropic associations of allelic variants in a 2q22 region with risks of major human diseases and mortality [16]. Given that the evolvability of APs protofibrils may act as epigenetic [1], it is predicted that the interaction of both genetics and epigenetics might be necessary to facilitate antagonistic pleiotropy in evolvability and neurodegeneration.

Fig.1

Schematics of the pathophysiology of APs in human brain. Evolvability is supposed to be a physiological phenomenon during reproduction, whereas neurodegenerative diseases are pathological phenomena during the post-reproductive senescent period. Both are derived from the aggregates of APs and participate in an antagonistic pleiotropy relationship as illustrated.

Schematics of the pathophysiology of APs in human brain. Evolvability is supposed to be a physiological phenomenon during reproduction, whereas neurodegenerative diseases are pathological phenomena during the post-reproductive senescent period. Both are derived from the aggregates of APs and participate in an antagonistic pleiotropy relationship as illustrated.

Our hypothesis may provide further insight into mechanisms of neurodegenerative disease that have been difficult to resolve by conventional theories of neurodegeneration. Recently, characterization of genetic mutations in PD, especially in terms of mitochondrial dysfunction and proteasomal neurotoxic activity, has greatly improved our understanding of the basis of familial PD [17]. Yet, the pathological mechanisms underlying sporadic PD, especially early disease pathogenesis, remain obscure. Our concept based on evolvability as a normal biological function during reproduction, postulates the preexistence of AP oligomers derived from parental brains which encode neuronal survival information passed to offspring [1]. To expand on this, during reproductive life stages, αS aggregation might be tightly regulated as a function of mitochondrial activity and ubiquitin-proteasome system. In turn, during aging, neurodegeneration could result from mitochondrial and ubiquitin- proteasomal dysfunction resulting in αS aggregation and neurotoxicity. Thus, in this instance, both evolvability and PD may represent a form of antagonistic pleiotropy manifested through aggregates of αS.

Our hypothesis may also account for the antagonistic pleiotropy of apolipoprotein E (ApoE), a major risk factor for AD [18]. The gene encoding for ApoE is polymorphic with three major alleles, ϵ2, ϵ3, and ϵ4, among which the ϵ3 allele is the most common with regard to the general population [19]. The presence of one or more ϵ4 alleles greatly increases risk of sporadic AD, whereas ϵ2 allele reduces risk and delays disease onset [19]. Despite association of the ɛ4 (ApoE4) allele with sporadic AD in aging, ApoE4 has persisted through evolution against the pressures of natural selection, indicating that ApoE4 might be biologically beneficial during reproduction. Provided that ApoE4 binds to Aβ and stimulates fibrillization [20], one might speculate that the evolvability of Aβ might be enhanced by ApoE4.

In addition, neuroinflammation may be regarded as another example of the antagonistic pleiotropy caused by APs. It has been well characterized that dysregulation of neuroinflammation by APs may result in production of high levels of pro-inflammatory and cytotoxic mediators, leading to exacerbation of neurodegenerative diseases, including PD and AD [21]. However, neuroinflammation is a double-edged sword [22]. During the reproduction period in humans, neuroinflammation may be beneficial for the protection against various neuronal injuries, such as infection, toxic chemicals and physical insults [22].

Given the existence of prions in microorganisms such as yeast [6], evolvability may represent an ancient phenomenon in the course of evolution. In contrast, ample evidence suggests that neurodegenerative disease may be a relative latecomer in evolution. Although neurodegeneration is rapidly induced in the brains of spawning salmon [23], such a phenomenon may be a physiological strategy to supply nutrients to offspring. Furthermore, it is noteworthy that several neuropathologic studies in the brains of aged nonhuman primates, including chimpanzee and gorillas, demonstrated amyloid senile plaques and neurofibrillary tangles, although it is unclear whether such lesions are associated with a behavioral phenotype [24–26]. Notably, it has been shown that post-menopausal senescence of the animals may be affected by various factors, such as captivity and kinship dynamics [27]. Thus, it is possible that the presence of plaques and tangles in other primates might be an artificial phenomenon due to the prolonged length of post-menopausal senescence under the capturing conditions. In this context, neurodegenerative diseases might be associated with an extended postmenopausal lifespan, which is specific to human beings [28, 29]. Taken together, we predict that the evolvability might be mechanistically situated upstream of neurodegenerative diseases and that their combined role in generating antagonistic pleiotropy may likely be a relatively recent phenomenon in human brain because of our extended postmenopausal lifespan.

In summary, evolvability of APs during reproduction may be biologically important and evolvability and aging-associated neurodegenerative diseases, such as PD and AD, might be an antagonistic pleiotropy relationship mediated by aggregates of APs. Our view might provide a broader perspective with which to view the entire spectrum of sporadic neurodegenerative diseases, enabling possibility of identifying novel therapeutic strategies for such conditions. Certainly, continued investigation is warranted to strengthen this intriguingpossibility.

Conflict of Interest

The authors have no conflict of interest to report.

ACKNOWLEDGMENTS

We are grateful for the continuous encouragement of Drs. Kaori Hashimoto (Tokyo Metropolitan Institute of Medical Science) and Maria del Carmen Ruiz de la Cruz (University of Chicago).

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