Banner Sun Health Research Institute, Sun City, AZ, USA
| [b] Inserm, U913, Nantes, France
Nantes University, Nantes, France
| [d] Department of Neurology, CHU Nantes, Nantes, France
| [e] CHU Angers, Neurobiology and Neuropathology Laboratory, Angers, France
Université of Angers, UPRES EA3143, Angers, France
| [g] Center for Brain Repair, Department of Pathology, Rush Medical College, Chicago, IL, USA
| [h] Roche Pharmaceutical Research and Early Development, Nord DTA, Biomarker and Clinical Imaging, Roche Innovation Center, F Hoffman-La Roche, Ltd., Basel | [i] Laboratory Medicine, St. Michael’s Hospital, University of Toronto & Li Ka Shing Knowledge Institute, Toronto, ON, Canada
| [j] Mayo Clinic Arizona, Scottsdale, Arizona
Correspondence to: Thomas G. Beach, MD, PhD, Banner Sun Health Research Institute, 10515 West Santa Fe Drive, Sun City, AZ 85351, USA. Tel.: +1 623 832 5643; Fax: +1 623 832 2967; E-mail: [email protected].
Abstract: Background: Conflicting results from studies of Lewy-type α−synucleinopathy (LTS) in colonic biopsies of subjects with Parkinson’s disease (PD) prompted a two-part multicenter assessment. The first assessment, now published (Acta Neuropathol Commun 4 : 35, 2016), examined archived colonic biopsies and found that none of the tested methods was adequately sensitive or specific. Objective: As the amount of nervous tissue in typical colonic biopsies may be insufficient, and the clinical diagnosis of PD not completely accurate, the objective of the current study was to use instead full-thickness sections of sigmoid colon from autopsy-proven PD and normal subjects. Methods: Seven different immunohistochemical (IHC) methods were used, employing five different primary antibodies and four different combinations of epitope exposure and signal development protocols. Specific staining was defined as being restricted to morphological features consistent with neuronal elements. Stained slides from each subject were independently categorized as being positive or negative for LTS, and their density semi-quantitatively graded, by four raters blinded to diagnosis. Results: Agreement and mean diagnostic performance varied markedly between raters. With the two most accurate raters, 5 methods achieved diagnostic accuracies of 70% or greater; one method had 100% accuracy and 100% inter-rater agreement. The submucosa had the highest prevalence of pathological LTS staining, followed by the muscularis and mucosa. Conclusions: The major conclusion of this study is that, when sufficient submucosa and LTS is present, and when specific staining is defined as being consistent with neuronal morphology, adequately-trained raters may reliably distinguish PD colon from control using suitable IHC methods.