Affiliations: Key Laboratory of Yangtze Water Environment of
Ministry of Education, Tongji University, Shanghai 200092, China | School of Life Science, Fudan University, Shanghai
200433, China | State Key Laboratory of Pollution Control and Resource
Reuse,Tongji University, Shanghai 200092, China | Department of Civil, Architectural and Environmental
Engineering, University of Missouri-Rolla, Rolla, MO 65409, USA
Abstract: An aerobic bacterium strain, F-3-4, capable of effectively degrading
2,6-di-tert-butylphenol (2, 6-DTBP), was isolated and screened out from an
acrylic fiber wastewater and the biofilm in the wastewater treatment
facilities. This strain was identified as Alcaligenes sp. through
morphological, physiological and biochemical examinations. After cultivation,
the strain was enhanced by 26.3% in its degradation capacity for 2,6-DTBP.
Results indicated that the strain was able to utilize 2,6-DTBP, lysine,
lactamine, citrate, n-utenedioic acid and maiic acid as the sole carbon and
energy source, alkalinize acetamide, asparagine, L-histidine, acetate, citrate
and propionate, but failed to utilize glucose, D-fructose, D-seminose, D-xylose,
serine and phenylalanine as the sole carbon and energy source. The optimal
growth conditions were determined to be: temperature 37°C, pH 7.0,
inoculum size 0.1% and shaker rotary speed 250 r/min. Under the optimal
conditions, the degradation kinetics of 2,6-DTBP with an initial concentration
of 100 mg/L was studied. Results indicated that 62.4% of 2,6-DTBP was removed
after 11 d. The degradation kinetics could be expressed by Eckenfelder equation
with a half life of 9.38 d. In addition, the initial concentration of 2,6-DTBP
played an important role on the degradation ability of the strain. The maximum
initial concentration of 2,6-DTBP was determined to be 200 mg/L. Above this
level, the strain was overloaded and exhibited significant inhibition.
