Construction of hybrid cell with Phanerochaete chrysosporium todegrade terephthalic acid wastewater (I) phenotype evidence

Article type: Research Article

Authors: Cheng, Shu-pei | Zhong, Zhi-hui | Lu, Peng | Chen, Jun | Zhu, Jian-zhong | Jue, Zi-long

Affiliations: The National Key Laboratory of Pollution Control and Resource Reuse, Department of Environmental Sciences and Engineering,Nanjing University, Nanjing 210093, China | Water Plant, Yangzi Petrochemical Corporation, Nanjing 210048, China

Note: [] Corresponding author

Abstract: The fungi Phanerochaete chrysosporium (PC) and Saccharomyces cerevisiae Y99 and the native bacterium YZ1 were the three parental strains for construction of hybrid cells through protoplast fusion to degrade terephthalic acid (TPA) wastewater. The results showed that the native bacterium YZ1 protoplasm could integrate with that of PC to form the hybrid cell Fhh and the fungus Y99 protoplasm also could integrate with that of Fhh to form the hybrid cell Fhhh. The protoplasts of YZ1 and Y99 could change the morphology of PC spore and mycelinm for two times. The hybrid cell Fhhh got the best growth and degradation abilities in the wastewater. It suggested that the hybrid strains obtained from the inter-kingdom protoplast fusion of the three parental strains could create potential for the purification of TPA wastewater.

Keywords: YZ1 bacterium, Saccharomyces cerevisiae, protoplast fusion, terephthalic acid wastewater, Phanerochaete chrysosporium

Journal: Journal of Environmental Sciences, vol. 12, no. 4, pp. 394-397, 2000