Bet v 1 potential allergens are involved in anthracnose resistance of strawberry varieties

Article type: Research Article

Authors: Yang, Jing^a | Ding, Zhiwen^b | Wang, Jing^c | Tian, Shuhua^a | Duan, Ke^a | Gao, Qinghua^{a; *}

Affiliations: [a] Shanghai Key Laboratory of Protected Horticultural Technology, Forestry and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai, China | [b] Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institutes of Biomedical Sciences, Shanghai, China | [c] Department of Biostatistics, Vanderbilt University Medical Center, Nashville, Tennessee, USA

Correspondence: [*] Corresponding author: Qinghua Gao, Shanghai Key Laboratory of Protected Horticultural Technology, Forestry and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences (SAAS), 1000 Jinqi Rd., Shanghai, 201403, P. R. China. Tel.: +86 21 37195672; Fax: +86 21 37195702; E-mail: [email protected].

Abstract: BACKGROUND:Bet v 1 family identified as one major plant food allergen class, is highly homologous to pathogenesis-related protein 10 (PR-10), but its biological function involved in disease resistance is still unclear. OBJECTIVE:This study aims to investigate whether Bet v 1 potential allergens participate in the resistance of berry crops against fungal pathogen. METHODS:Allergenicity of Bet v 1 proteins in strawberry (Fragaria) was evaluated by bioinformatics methods. Their expression in response to anthracnose and between susceptible and resistance varieties was analyzed. RESULTS:19 Bet v 1 homologous proteins were identified and 15 of them were considered as allergen candidates. RNA-seq analysis indicated most of these Fra a 1s expressed in fruits could be largely induced by the invasion of anthracnose pathogen Colletotrichum. The mRNA level of fruit major allergen Fra a 1.05 in the resistant variety Shenyang (SY) was 20∼50 fold higher compared with those in the susceptible cultivar and two diploid wild species. Immunoblotting using Birch (Betula pendula) Bet v 1 allergen-specific IgG antibody confirmed the large-scale accumulation of potential cross-reactive antigens in SY fruit. CONCLUSIONS:Strawberry Bet v 1 potential allergens exhibit their correspondence with anthracnose resistance that might be instructive to future breeding strategies.

Keywords: Bet v 1, Fragaria; Colletotrichum , disease resistance, allergy

DOI: 10.3233/JBR-200627

Journal: Journal of Berry Research, vol. Pre-press, no. Pre-press, pp. 1-12, 2021