In vitro rooting and acclimatization of Amelanchier alnifolia (Nutt.) Nutt. ex M. Roem: Testing of auxin, spermidine, and gibberellin for overcoming dormancy
Affiliations: Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Center, Slovak Academy of Sciences, Nitra, Slovakia
Correspondence:
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Correspondence to: Alena Gajdošová, Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Center, Slovak Academy of Sciences, Akademická 2, 95007, Nitra, Slovakia. Tel.: +421 37 9643 315; Fax: +421 37 73 366 60; E-mail: [email protected].
Abstract: BACKGROUND:Amelanchier alnifolia is an attractive small fruit difficult to propagate by traditional methods. Moreover, this species can be susceptible to dormancy after transplanting to the soil. OBJECTIVE:The aim of this work was to evaluate factors that contribute to effective in vitro rooting and acclimatization of micropropagated shoots of Amelanchier alnifolia. METHODS:Different auxins, media concentrations, and sprays containing plant growth regulators were tested. The experimental data were treated by analysis of variance. RESULTS:1-naphthalene acetic acid (NAA) was superior for rooting compared to indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). Cultivation of shoots on Murashige & Skoog (MS) medium with a half-strength concentration (1/2 MS) led to higher rooting frequencies than on full-strength MS medium. Addition of 1.5 mg l–1 spermidine to cultivation medium did not significantly improve rooting. Gibberellic acid (GA4+7) alone or in combination with 6-benzylaminopurine (BAP) did not effectively break post-rooting dormancy. CONCLUSION:The greatest number of actively growing plants was recorded after rooting the shoots on 1/2 MS medium with 1 mg l–1 NAA followed by a spray treatment with 1 mg l–1 BAP. These results are directly applicable for improving rooting efficiency and acclimatization of micropropagated Amelanchier spp. plantlets.
