Evaluation of strawberry resistance to crown rot caused by Phytophthora cactorum is usually performed on a limited number of genotypes. The major objective of this study was to screen large genetic resources to identify potential parents that can be further used in breeding programs. Plants were inoculated by wounding the crown and placing a mycelium disk on the wound. Firstly, plug and cold stored plants were evaluated for their susceptibility to P. cactorum. Secondly, a total of 107 genotypes was evaluated using cold stored plants. Plug plants were very low affected by the wounding inoculation with P. cactorum whatever the genotype, whereas results obtained using cold stored plants consistently reflected the susceptibility of the genotype to crown rot. By using cold stored plants, we evaluated the susceptibility of 70 varieties and 37 advanced lines. Among the genotypes, we identified varieties such as Cirafine or Cireine with high level of resistance similar to the one of Senga Sengana. These data will be useful for choosing parents in breeding programs and for validation of markers linked to the resistance to P. cactorum.