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Superoxide anion radical scavenging activity of bilberry (Vaccinium myrtillus L.)

Article type: Research Article

Authors: Tumbas, Vesna | Čanadanović-Brunet, Jasna | Gille, Lars | Đilas, Sonja | Ćetković, Gordana

Affiliations: Faculty of Technology, University of Novi Sad, Novi Sad, Serbia | Molecular Pharmacology and Toxicology Unit, Department of Biomedical Sciences, University of Veterinary Medicine Vienna, Vienna, Austria

Note: [] Corresponding author: V. Tumbas, Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia. Tel.: +381 21 485 3652; Fax: +381 21 450 413; E-mail: [email protected]

Keywords: Bilberry, ESR spectroscopy, superoxide anion radicals, phenolic acids, flavonoids, vitamin C

DOI: 10.3233/BR-2010-002

Journal: Journal of Berry Research, vol. 1, no. 1, pp. 13-23, 2010

Published: 2010
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Abstract

Naturally occurring antioxidants present in bilberry were separated into three groups: vitamin C, phenolic acids and flavonoids using solid phase extraction (SPE). Chemical composition of bilberry extract fractions was obtained by spectrophotometry and high performance liquid chromatography (HPLC). The results of HPLC analysis point out the high content of vitamin C (1529 μg/g) in fraction Fr1, flavonoids (1328.58 μg/g) in fraction Fr2 and phenolic acids (494.31 μg/g) in fraction Fr3. The free radical scavenging activities of these antioxidant fractions on superoxide anion radicals and detection of free radical intermediates was studied using electron spin resonance (ESR) spectroscopy. Superoxide anion free radical assay demonstrated very potent free radical scavenging activity of bilberry extract fractions. The results of correlation analysis showed that the separated classes of antioxidants from bilberry (vitamin C, flavonoids and phenolic acids) are responsible for its antioxidant activity.

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