NB-02 Protects Neurons and Astrocytes from Oligomeric Amyloid-β-Mediated Damage

Article type: Short Communication

Authors: Miller, Morgan R. | Lariviere, Lavender | Pagnier, Guillaume J. | Aygar, Sema | Wieckiewicz, Natalia | Maesako, Masato | Bacskai, Brian J. | Kastanenka, Ksenia V.^{; *}

Affiliations: [a] Department of Neurology, MassGeneral Institute of Neurodegenerative Diseases, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, USA

Correspondence: [*] Correspondence to: Ksenia V. Kastanenka, MassGeneral Institute of Neurodegenerative Diseases, 114 16th St. Charlestown, MA, 02129, USA. E-mail: [email protected].

Abstract: Alzheimer’s disease (AD) is a progressive neurodegenerative disease with limited therapeutic strategies. NB-02 is a novel botanical drug that has shown promise as a protective and therapeutic treatment for AD in an APP/PS1 preclinical mouse model. In this paper, we investigate the underlying mechanisms by which NB-02 provides these therapeutic advantages using in vitro neuron-astrocyte co-cultures. Pretreatment with NB-02 prevented pathological calcium elevations in neurons and astrocytes after application of toxic soluble amyloid-β (Aβ) oligomers. NB-02 also prevented cell death associated with the addition of soluble Aβ oligomers suggesting NB-02 is effective at protecting both neurons and astrocytes from Aβ-mediated damage.

Keywords: Alzheimer’s disease, amyloid, astrocyte, calcium, DA-9803, NB-02, neuron, oligomer, therapy

DOI: 10.3233/JAD-231387

Journal: Journal of Alzheimer's Disease, vol. 99, no. 2, pp. 477-483, 2024