Affiliations: [a] Key Laboratory of Atherosclerosis in Universities of Shandong; Institute of Atherosclerosis, Taishan Medical University, Taian, China | [b] School of Pharmaceutical Science, Taishan Medical University, Taian, China | [c] Department of Anatomy and Cell Biology, SUNY Downstate Medical Center, New York, USA
Correspondence:
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Correspondence to: Shucun Qin, Key Laboratory of Atherosclerosis in Universities of Shandong, Institute of Atherosclerosis, Taishan Medical University, Taian 271000, China. E-mail: [email protected].
Note: [1] These authors contributed equally to this work.
Abstract: Increased expression of phospholipid transfer protein (PLTP) was observed in the brains of Alzheimer's disease (AD) patients; however, the role of PLTP in the progress of AD is still poorly understood. The objective of this study was to evaluate the effect of PLTP deficiency on the recognition and metabolism of amyloid-β (Aβ) in mice. We performed the Morris water maze to determine the learning and memory capabilities of 50-week age wild type mice (WT, n = 12) and PLTP knockout mice (PLTP−/−, n = 12). The levels of Aβ and amyloid-β protein precursor (AβPP) were examined by ELISA and western blot, respectively. The levels and activity of β- and γ-secretases were determined by western blot and activity assay kit, respectively. Morris water maze results showed that PLTP deficiency significantly impaired recognition compared with WT mice. Levels of Aβ42 in the cortex and hippocampus was significantly increased, yet the levels of Aβ40 in the cortex was decreased in PLTP−/− compared with WT mice. No typical senile plaques were found in the WT or PLTP−/− mice. AβPP expression and β-secretase activity were both significantly increased in PLTP−/− mice. Moreover, PLTP deficiency increased the expression of γ-secretase catalytic units and decreased the content of apolipoprotein E. Therefore we concluded that PLTP deficiency impaired cognition and aggravated AD by enhancing the generation of Aβ in the cortex of old mice.
Keywords: Amyloid-β, amyloid-β protein precursor, capabilities of learning and memory, phospholipid transfer protein
