Affiliations: [a] Scuola Normale Superiore, Pisa, Italy | [b] Department of Biochemical Sciences and Istituto Pasteur – Fondazione Cenci Bolognetti, Sapienza University of Rome, Italy | [c] European Brain Research Institute – “Rita Levi-Montalcini”, Rome, Italy | [d] CNR Institute of Molecular Biology and Pathology, Rome, Italy
Correspondence:
[*]
Correspondence to: Antonino Cattaneo, Scuola Normale Superiore, Piazza dei Cavalieri 7, 56126 Pisa, Italy. Tel.: +39 50 509320; Fax: +39 50 3153210; E-mail: [email protected].
Note: [1] These authors contributed equally to this work.
Abstract: The 7WD4 and 7PA2 cell lines, widely used as cellular models for Alzheimer's disease (AD), have been used to investigate the effects of amyloid-β protein precursor overexpression and amyloid-β (Aβ) oligomer accumulation on mitochondrial function. Under standard culture conditions, both cell lines, compared to Chinese hamster ovary (CHO) control cells, displayed an ~5% decrease of O2 respiration as sustained by endogenous substrates. Functional impairment of the respiratory chain was found distributed among the protein complexes, though more evident at the level of complex I and complex IV. Measurements of ATP showed that its synthesis by oxidative phosphorylation is decreased in 7WD4 and 7PA2 cells by ~25%, this loss being partly compensated by glycolysis (Warburg effect). Compensation proved to be more efficient in 7WD4 than in 7PA2 cells, the latter cell line displaying the highest reactive oxygen species production. The strongest deficit was observed in mitochondrial membrane potential that is almost 40% and 60% lower in 7WD4 and 7PA2 cells, respectively, in comparison to CHO controls. All functional parameters point to a severe bioenergetic impairment of the AD cells, with the extent of mitochondrial dysfunction being correlated to the accumulation of Aβ peptides and oligomers.
