Affiliations: [a] Laboratory of Cellular & Molecular Neurosciences, Faculty of Sciences, University of Chile, Santiago, Chile | [b] International Center for Biomedicine (ICC), Santiago, Chile | [c] Department of Neurological Sciences, Faculty of Medicine, University of Chile, Santiago, Chile | [d] Cognitive Neurology and Dementia Unit, Neurology Department, Hospital del Salvador, Santiago, Chile
Correspondence:
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Correspondence to: Prof. Dr. R.B. Maccioni, Director LCMN, Faculty of Sciences, University of Chile, Las Encinas 3370, Ñuñoa, Santiago, Chile. E-mail: [email protected].
Note: [1] These authors contributed equally.
Abstract: Platelets are a major peripheral reservoir of the amyloid-β protein precursor, so they have been considered as a potential biological marker of Alzheimer's disease (AD). Here, it is demonstrated that tau protein is also present in platelets and that the levels of oligomeric species of this protein could serve as a novel and reliable biological marker for AD. Blood samples were obtained from 15 AD patients and 10 paired-age controls and platelets were separated via differential centrifugation. The purity of platelets was determined by flow cytometry and microscopy and the presence of tau was determined by immunofluorescence and immunoblots with tau specific antibodies. Immunofuorescence and immunoblot patterns of platelets were positive for tau. Immunoblots also showed the presence of high molecular weight (HMW) variants of tau that appeared to correspond to oligomeric forms of the protein. The ratio of HMW tau respect to tau monomeric species was significantly higher in AD patients than controls. The present is the first description of the presence of tau in platelets. The analysis of different tau fractions in platelets could serve as a new biological marker for AD.
Keywords: Alzheimer's disease, cognitive impairment, human platelets, molecular biomarkers, protein self aggregation, tau proteins
