A Method for the Detection of Amyloid-β_1-40, Amyloid-β_1-42 and Amyloid-β Oligomers in Blood Using Magnetic Beads in Combination with Flow Cytometry and its Application in the Diagnostics of Alzheimer's Disease

Article type: Short Communication

Authors: Santos, Alexander Navarrete^{a; *} | Simm, Andreas^a | Holthoff, Vjera^b | Boehm, Gerald^c

Affiliations: [a] Department of Cardiothoracic Surgery, Martin-Luther University Halle Wittenberg, Ernst Grube Straße 40, 06120 Halle, Germany | [b] Department of Psychiatry and Psychotherapy, University of Technology, Fetscherstrasse 74, 01307 Dresden, Germany | [c] IGZ Innovation and Founding Center Würzburg BioMed/ZmK, Friedrich-Bergius-Ring 15, 97076 Würzburg, Germany

Correspondence: [*] Corresponding author: Alexander Navarrete Santos, Ernst Grube Straße 40, 06120 Halle, Germany. Tel.: +49 345 557 3314; Fax: +49 345 557 3317; E-mail: [email protected].

Note: [] Communicated by A. Cristina Rego.

Abstract: A method for simultaneous quantification of amyloid-β1-40, amyloid-β1-42 and amyloid-β oligomers in human plasma is described. The method consists of a combination of immunoprecipitation using specific antibodies against the different forms of amyloid-β, and immobilization of the immunocomplexes to magnetic beads. Addition of fluorescence-labelled antibodies which recognize the specific antibodies to the amyloid-β subsets allows the peptide/associates detection in the sample by flow cytometry. The clinical assay performance was tested using blood samples from Alzheimer disease's patients and control donors. A sensitivity of 70% and a specificity of 81% was achieved.

Keywords: Alzheimer's disease, amyloid-β, flow cytometry, human plasma, immunoprecipitation, magnetic beads

DOI: 10.3233/JAD-2008-14201

Journal: Journal of Alzheimer's Disease, vol. 14, no. 2, pp. 127-131, 2008