Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Kato, Masatoshia; | Mochizuki, Katsumia | Kuroda, Kazuhikoa | Sato, Susumua | Murakami, Hirokib | Yasumoto, Koseic | Nomoto, Kikuoc | Hashizume, Shuichia
Affiliations: [a] Morinaga Institute of Biological Science, Yokohama, Japan | [b] Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan | [c] Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
Note: [] Address reprint requests to: Dr. Masatoshi Kato, Morinaga Institute of Biological Science, 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama 230, Japan.
Abstract: Histone H2B was demonstrated to be an immunoreactive material recognized by the human monoclonal antibody HB4C5, which had been already established to be specific for lung cancers. The inhibitory effect of histone H2B on the activity of HB4C5 antibody to immunostain the cytoplasmic antigen in lung adenocarcinoma tissue indicated that histone H2B at least had antigenic determinant comparable to the cytoplasmic antigen. A mouse anti-histone H2B monoclonal antibody could immunostain the cytoplasm of lung adenocarcinoma cells in sliced tissue sections in the same manner as the human monoclonal antibody HB4C5. Enzyme-linked immunosorbent assay of HB4C5 antibody on plastic immunoplates coated with histone H2B also showed specific reactivity of this antibody with histone H2B, and the reaction was effectively inhibited when extra histone H2B or mouse anti-histone H2B monoclonal antibody was added to the reaction mixture. These results consistently lead us to a conclusion that histone H2B possesses antigenicity to the human monoclonal antibody HB4C5.
Keywords: histone H2B, cancer marker, lung adenocarcinoma, human monoclonal antibody
DOI: 10.3233/HAB-1991-2207
Journal: Human Antibodies, vol. 2, no. 2, pp. 94-101, 1991
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
[email protected]
For editorial issues, like the status of your submitted paper or proposals, write to [email protected]
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
[email protected]
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office [email protected]
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
[email protected]
For editorial issues, like the status of your submitted paper or proposals, write to [email protected]
如果您在出版方面需要帮助或有任何建, 件至: [email protected]