Affiliations: Department of Histopathology, Kings College
London, Kings Denmark Hill Campus, London, United Kingdom
Note: [] Address for correspondence: J.R. Salisbury Department of
Histopathology Kings College LondonKings Denmark Hill
CampusLondon, United Kingdom
Abstract: Three-dimensional reconstructions producing computer-generated false
colour images have played an important role in furthering understanding in many
areas in microscopical morphology, both in anatomy and pathology. This review
article considers several recent advances made in the understanding of tumour
pathology by the use of these computer-generated three-dimensional models.
However, three-dimensional reconstructions can be made at a number of levels of
resolution - at the level of a whole tissue or organ, at the level of cells or
part of a tissue, and at the subcellular level. Examples of three-dimensional
reconstructions made at each of these levels of resolution are discussed, with
reference to human fetal notochords, bone marrow trephine specimens, and
nucleoli from human fibroblasts, Spirogyra grevilleana, mouse Sertoli cells,
and pea meristematic cells.
