Affiliations: Biochemistry Department, Faculty of Medicine, Ain
Shams University, Cairo, Egypt | Oncolgy Diagnostic Unit, Faculty of Medicine, Ain
Shams University, Cairo, Egypt | Biochemistry Department, Faculty of Science, Ain Shams
University, Cairo, Egypt | Urology Department, Faculty of Medicine, Ain Shams
University, Cairo, Egypt
Note: [] Corresponding author: Sanaa Eissa, Medical Biochemistry and
Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo,
Egypt. E-mail: [email protected]
Abstract: Objective: In a trial to improve the diagnostic efficacy of
conventional urine cytology we determine survivin RNA and matrix
metalloproteinase 2 and 9 in urine of bladder cancer cases. Method: Voided urine specimens were collected from patients with
histologically confirmed bladder urothelial carcinoma (Group 1; n=46),
urological patients without urothelial carcinoma (Group 2; n=20), and healthy
volunteers (Group 3; n=20). Urine cytology, survivin RNA was estimated by
qualitative nested RT-PCR and MMP-2, MMP-9 activity were detected by gelatin
zymography. The expression of survivin RNA and matrix metalloproteinase 2 and 9
in bladder cancer was compared with benign and normal cases. Results: Positivity rates of survivin RNA and MMPs zymography were
significantly different among the 3 groups. Urine survivin detection by
qualitative nested RT-PCR showed 76.1% sensitivity and 95% specificity. The
overall sensitivity, specificity of urinary MMP zymography was 67.3%, 90%
respectively. The combined use of urine cytology with urine survivin or MMPs
zymography increased sensitivity of urine cytology from 50% to 84.7%. The
highest sensitivity (95.6%) was obtained on combining the three markers. Conclusion: Survivin RNA and MMPS zymography can be considered as
promising noninvasive markers for bladder cancer early detection. Combined use
of the three markers improved the sensitivity for detecting bladder cancer.
