Affiliations: División de Medicina Molecular, Centro de
Investigación Biomédica de Occidente, Instituto Mexicano del Seguro
Social, Guadalajara, Jalisco, México | Instituto de Investigación en Reumatología y
del Sistema Músculo Esquelético, Centro Universitario de Ciencias de la
Salud, Universidad de Guadalajara, Jalisco, México | Hospital General de Zona No. 1, Instituto Mexicano del
Seguro Social, Colima, Colima, México | Coordinación Delegacional de Investigación en
Salud, Instituto Mexicano del Seguro Social, Tepic, Nayarit, México
Note: [] Corresponding author: Silvia Esperanza Flores-Martínez,
Ph.D., División de Medicina Molecular, Centro de Investigación
Biomédica de Occidente, Instituto Mexicano del Seguro Social. Sierra Mojada
800, Col. Independencia, C.P. 44340, Guadalajara, Jalisco, México. Tel.:
+52 3336 189410; Fax: +52 3336 181756; E-mail: [email protected] and
[email protected]
Abstract: The {association} between some Tumor necrosis factor-alpha
(TNF-α) promoter polymorphisms and Type 2 diabetes
mellitus (T2DM) remains controversial. Ethnic differences may play a role in
these conflicting results. The aim of this study was to investigate the
association between −308G/A and −238G/A
polymorphisms located in the promoter region of the TNF-α gene and T2DM in Mexican mestizo patients. Nine hundred four
individuals (259 patients with T2DM and 645~controls) were genotyped for the
−308G/A and −238G/A polymorphisms by
PCR--RFLP. We found that the −238A allele increased the risk
of developing T2DM in Mexican patients (OR=1.57, 95%
CI: 1.07–2.29; p=0.018). Moreover, we found that the frequency of the GA
haplotype (created by the −308G and −238A
alleles) was significantly increased in patients with T2DM when compared with
controls (OR =1.56, 95% CI: 1.05–2.31; p=0.026). Our
results suggest that the −238G/A polymorphism and a specific
haplotype (GA) are genetic risk factors for the development of T2DM in Mexican population.
Keywords: Haplotype, Mexican patients, polymorphism, TNF-alpha, Type 2 diabetes mellitus
