Affiliations: Department of Periodontology,Gaziantep University
Faculty of Dentistry, 27310 Gaziantep, Turkey | Department of Medical Biology and Genetics, Gaziantep
University Faculty of Medicine, 27310 Gaziantep, Turkey | Department of Periodontology, Ataturk University
Faculty of Dentistry, 25240 Erzurum, Turkey
Note: [] Corresponding author: Kamile Erciyas, Gaziantep University
Faculty of Dentistry, Department of Periodontology, Gaziantep, TR-27310,
Turkey. Tel.: +90 342 360 60 60 (76611); Fax: +90 342 361 03 46; E-mail:
[email protected], [email protected]
Abstract: Myeloperoxidase (MPO) is a lysosomal enzyme found in the azurophilic
granules of polymorphonuclear leukocytes. It is involved in the defense against
periodontal bacteria, and is also able to mediate inflammatory tissue
destruction in aggressive and chronic periodontitis. The aim of this study was
to explore the association between MPO-463G/A gene polymorphism and aggressive
periodontitis (AgP) and chronic periodontitis (CP). The study included 147
subjects. Probing depth (PD), clinical attachment loss (CAL), plaque index
(PI), and gingival index (GI) were recorded as the clinical parameters. Genomic
DNA was obtained from the peripheral blood of 32 subjects with AgP, 25 with CP,
and 90 reference controls. We genotyped the MPO-463G/A polymorphism using the
PCR-RFLP method. All data were analyzed using SPSS version 13.0 for windows.
There were no significant differences between the CP patients and controls
regarding MPO-463A/G gene polymorphism either in terms of allele frequency or
genotype frequency of MPO-463A/G. However, either in terms of allele frequency
or genotype frequency of MPO-463A/G, there were significant differences between
the AgP patients and the controls. In conclusion, our data suggest that
MPO-463G/A may be associated with increased risk of aggressive periodontitis in
Turkish patients.
