Article type: Research Article
Authors: Ortiz, G.G. | Pacheco-Moisés, F. | El Hafidi, M. | Jiménez-Delgado, A. | Macías-Islas, M.A. | Corral, S.A. Rosales | de la Rosa, A. Célis | Sánchez-González, V.J. | Arias-Merino, E.D. | Velázquez-Brizuela, I.E.
Affiliations: Laboratorio Desarrollo-Envejecimiento, Enfermedades
Neurodegenerativas, Division Neurociencias, Centro de Investigación
Biomédica de Occidente (CIBO), Instituto Mexicano de Seguro Social (IMSS),
Guadalajara, Jalisco, México | Centro Universitario de Ciencias Exactas e
Ingeniería (CUCEI), Universidad de Guadalajara, Guadalajara, Jalisco,
México | Departamento de Bioquímica, Instituto Nacional de
Cardiología, México | Departamento de Neurología, UMAE-HE-CMNO, IMSS,
Guadalajara, Jalisco, México | Departamento de Salud Pública, Centro
Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara,
Jalisco, México
Note: [] Corresponding author: Genaro Gabriel Ortiz, MD, PhD, Laboratorio
Desarrollo-Envejecimiento, Enfermedades Neurodegenerativas, CIBO-IMSS, Sierra
Mojada 800 CP 44340, Guadalajara, Jalisco, México. Tel.: +52 33 3638 5593;
E-mail: [email protected]
Abstract: It has been suggested that mitochondrial dysfunction and defects in
membrane structure could be implied in AD pathogenesis. The aim of the present
work was the study of membrane fluidity in submitochondrial platelet particles
and erythrocyte membranes from Mexican patients. Blood samples were obtained
from 30 patients with Alzheimer disease and 30 aged-matched control subjects.
Membrane fluidity determinations were done using a very low concentration of
the fluorescent dipyrenylpropane probe incorporated in both types of membranes.
This probe is able to give excimer and monomer fluorescence, therefore it can
be used to monitor fluidity changes in biological membranes. The data obtained
showed that in submitochondrial particles from AD patients, the excimer to
monomer fluorescent intensity ratio was lower (0.231 ±
0.008) than aged-matched control subjects (0.363 ± 0.014).
Therefore, membrane fluidity was lower in AD samples. On the other hand, we
found similar membrane fluidity in erythrocytes from AD patients and
aged-matched controls: the fluorescent intensity ratios were 0.312
± 0.03 and 0.305 ± 0.033,
respectively. In addition, lipid peroxidation in submitochondrial particles and
erythrocyte membranes was higher in AD samples than in aged-matched controls.
These data suggest that submitochondrial platelet particles are more sensitive
to oxidative stress than erythrocyte membranes.
Keywords: Membrane fluidity, Alzheimer disease, platelets, mitochondria, dipyrenylpropane
Journal: Disease Markers, vol. 24, no. 3, pp. 151-156, 2008
Received 28 February 2008
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Accepted 28 February 2008
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Published: 2008
